Skip to content

Latest commit

 

History

History

SNP genotyping and Detection of eQTL

SNP genotyping of F5 lines

RNA-seq data necessary to run the scripts are available on SRA under the BioProject ID PRJNA1128551.

Scripts:

trimPC/PW60.pl - performs trimming of the RNA-seq data

hisatPC/PW.pl - performs mapping of trimmed RNa-seq data to the genome reference

picard-markdup-index-PC/PW.pl - performs preparation of bamfiles for variant calling

haplotype-caller-PC/PW.pl - performs haplotype calling on individiual samples

gatk-genotypegvcfs.PC/PW.pl - performs joint genotyping on samples

Detection of eQTL

Data tables necessary to run the scripts are available on figshare

Scripts:

eQTL_analysis_phenotype_data_incl.R - runs the SNP filtering and subsequent eQTL analysis