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Implement QC functions for profiling #2

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shntnu opened this issue Feb 28, 2017 · 3 comments
Open

Implement QC functions for profiling #2

shntnu opened this issue Feb 28, 2017 · 3 comments

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@shntnu
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shntnu commented Feb 28, 2017

The purpose of this issue is to create a list. Once we settle on a list, we will close the issue and create an issue per QC item. We also need to decide where to implement this – here or in http://github.com/CellProfiler/cytominer

  • Plot illumination corrections functions
  • Plot salient features on a plate map to see if there are any trends
    • Cell count
    • IntegratedIntensity
    • PercentMaximal
  • Show excluded wells on a plate map
  • Check for rotation of the plate layout
    • Plate map with cell counts
    • Cluster all the wells across plates
@shntnu
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shntnu commented Mar 8, 2017

For each plate show the signature strength/reproducibility metric as a heatmap, so that you can see whether bad/good samples fall on certain plates or certain regions of plates
This could also help diagnose metadata problems

@mrohban
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mrohban commented Aug 25, 2017

heat map plot for the locations of 2N and 4N peaks in the DNA integrated intensity would help to find out how the segmentation worked

@shntnu
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shntnu commented Feb 6, 2021

image

Histogram of all features from a single well – this one had a few outlier features, post normalization. Should we flag these wells?

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