diff --git a/01-getting-started-with-nextflow.html b/01-getting-started-with-nextflow.html index 7c26970f..dc3c83b6 100644 --- a/01-getting-started-with-nextflow.html +++ b/01-getting-started-with-nextflow.html @@ -667,7 +667,7 @@
You should see output similar to the text shown below:
Another useful factory method is fromSRA
. The
-fromSRA
method makes it possible to query the NCBI SRA archive and returns
-a queue channel emitting the FASTQ files matching the specified
-selection criteria.
The queries can be project IDs or accession numbers supported by the -NCBI -ESearch API.
-If you want to use this functionality, you will need an NCBI
-API KEY, and to set the environment variable
-NCBI_API_KEY
to its value.
This will print a tuple for every fastq file associated with that SRA -project accession.
-[SRR1448794, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/004/SRR1448794/SRR1448794.fastq.gz]
-[SRR1448795, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/005/SRR1448795/SRR1448795.fastq.gz]
-[SRR1448792, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/002/SRR1448792/SRR1448792.fastq.gz]
-[SRR1448793, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/003/SRR1448793/SRR1448793.fastq.gz]
-[SRR1910483, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/003/SRR1910483/SRR1910483.fastq.gz]
-[SRR1910482, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/002/SRR1910482/SRR1910482.fastq.gz]
-(remaining omitted)
-Multiple accession IDs can be specified using a list object:
-ids = ['ERR908507', 'ERR908506', 'ERR908505']
-sra_ch = Channel.fromSRA(ids)
-sra_ch.view()
-[ERR908507, [ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908507/ERR908507_1.fastq.gz, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908507/ERR908507_2.fastq.gz]]
-[ERR908506, [ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908506/ERR908506_1.fastq.gz, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908506/ERR908506_2.fastq.gz]]
-[ERR908505, [ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908505/ERR908505_1.fastq.gz, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908505/ERR908505_2.fastq.gz]]
-Use the --help
flag to print the list command usage.
Use the nextflow pull
command to download the latest
nf-core/rnaseq
pipeline
A line similar to one below in the file custom.config
params.email = "myemail@address.com"
@@ -1156,7 +1156,7 @@ You should see output similar to the text shown below:
Another useful factory method is fromSRA
. The
-fromSRA
method makes it possible to query the NCBI SRA archive and returns
-a queue channel emitting the FASTQ files matching the specified
-selection criteria.
The queries can be project IDs or accession numbers supported by the -NCBI -ESearch API.
-If you want to use this functionality, you will need an NCBI
-API KEY, and to set the environment variable
-NCBI_API_KEY
to its value.
This will print a tuple for every fastq file associated with that SRA -project accession.
-[SRR1448794, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/004/SRR1448794/SRR1448794.fastq.gz]
-[SRR1448795, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/005/SRR1448795/SRR1448795.fastq.gz]
-[SRR1448792, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/002/SRR1448792/SRR1448792.fastq.gz]
-[SRR1448793, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/003/SRR1448793/SRR1448793.fastq.gz]
-[SRR1910483, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/003/SRR1910483/SRR1910483.fastq.gz]
-[SRR1910482, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/002/SRR1910482/SRR1910482.fastq.gz]
-(remaining omitted)
-Multiple accession IDs can be specified using a list object:
-ids = ['ERR908507', 'ERR908506', 'ERR908505']
-sra_ch = Channel.fromSRA(ids)
-sra_ch.view()
-[ERR908507, [ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908507/ERR908507_1.fastq.gz, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908507/ERR908507_2.fastq.gz]]
-[ERR908506, [ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908506/ERR908506_1.fastq.gz, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908506/ERR908506_2.fastq.gz]]
-[ERR908505, [ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908505/ERR908505_1.fastq.gz, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908505/ERR908505_2.fastq.gz]]
-Use the --help
flag to print the list command usage.
Use the nextflow pull
command to download the latest
nf-core/rnaseq
pipeline
A line similar to one below in the file custom.config
params.email = "myemail@address.com"
@@ -10021,7 +9960,7 @@ The nextflow scripts for each lesson episode are available in the
scripts
directory created during the course setup. You
@@ -689,7 +689,7 @@
You should see output similar to the text shown below:
Create a nextflow script called to channel.nf
for the
following examples. Explain that as you are just setting up channels you
@@ -679,7 +679,7 @@
Another useful factory method is fromSRA
. The
-fromSRA
method makes it possible to query the NCBI SRA archive and returns
-a queue channel emitting the FASTQ files matching the specified
-selection criteria.
The queries can be project IDs or accession numbers supported by the -NCBI -ESearch API.
-If you want to use this functionality, you will need an NCBI
-API KEY, and to set the environment variable
-NCBI_API_KEY
to its value.
This will print a tuple for every fastq file associated with that SRA -project accession.
-[SRR1448794, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/004/SRR1448794/SRR1448794.fastq.gz]
-[SRR1448795, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/005/SRR1448795/SRR1448795.fastq.gz]
-[SRR1448792, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/002/SRR1448792/SRR1448792.fastq.gz]
-[SRR1448793, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/003/SRR1448793/SRR1448793.fastq.gz]
-[SRR1910483, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/003/SRR1910483/SRR1910483.fastq.gz]
-[SRR1910482, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/002/SRR1910482/SRR1910482.fastq.gz]
-(remaining omitted)
-Multiple accession IDs can be specified using a list object:
-ids = ['ERR908507', 'ERR908506', 'ERR908505']
-sra_ch = Channel.fromSRA(ids)
-sra_ch.view()
-[ERR908507, [ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908507/ERR908507_1.fastq.gz, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908507/ERR908507_2.fastq.gz]]
-[ERR908506, [ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908506/ERR908506_1.fastq.gz, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908506/ERR908506_2.fastq.gz]]
-[ERR908505, [ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908505/ERR908505_1.fastq.gz, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908505/ERR908505_2.fastq.gz]]
-Use the --help
flag to print the list command usage.
Use the nextflow pull
command to download the latest
nf-core/rnaseq
pipeline
A line similar to one below in the file custom.config
params.email = "myemail@address.com"
@@ -1158,7 +1158,7 @@ The nextflow scripts for each lesson episode are available in the
scripts
directory created during the course setup. You
@@ -765,7 +765,7 @@
You should see output similar to the text shown below:
Create a nextflow script called to channel.nf
for the
following examples. Explain that as you are just setting up channels you
@@ -1535,7 +1535,7 @@
Another useful factory method is fromSRA
. The
-fromSRA
method makes it possible to query the NCBI SRA archive and returns
-a queue channel emitting the FASTQ files matching the specified
-selection criteria.
The queries can be project IDs or accession numbers supported by the -NCBI -ESearch API.
-If you want to use this functionality, you will need an NCBI
-API KEY, and to set the environment variable
-NCBI_API_KEY
to its value.
This will print a tuple for every fastq file associated with that SRA -project accession.
-[SRR1448794, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/004/SRR1448794/SRR1448794.fastq.gz]
-[SRR1448795, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/005/SRR1448795/SRR1448795.fastq.gz]
-[SRR1448792, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/002/SRR1448792/SRR1448792.fastq.gz]
-[SRR1448793, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR144/003/SRR1448793/SRR1448793.fastq.gz]
-[SRR1910483, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/003/SRR1910483/SRR1910483.fastq.gz]
-[SRR1910482, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/002/SRR1910482/SRR1910482.fastq.gz]
-(remaining omitted)
-Multiple accession IDs can be specified using a list object:
-ids = ['ERR908507', 'ERR908506', 'ERR908505']
-sra_ch = Channel.fromSRA(ids)
-sra_ch.view()
-[ERR908507, [ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908507/ERR908507_1.fastq.gz, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908507/ERR908507_2.fastq.gz]]
-[ERR908506, [ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908506/ERR908506_1.fastq.gz, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908506/ERR908506_2.fastq.gz]]
-[ERR908505, [ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908505/ERR908505_1.fastq.gz, ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR908/ERR908505/ERR908505_2.fastq.gz]]
-