From 534898ef998da1f2946ad95e6a1d89d16c7e5ebb Mon Sep 17 00:00:00 2001 From: Damien Marsic Date: Fri, 19 Jun 2020 09:53:14 +0800 Subject: [PATCH] update --- docs/_build/doctrees/environment.pickle | Bin 14751 -> 14924 bytes docs/_build/doctrees/pm-doc.doctree | Bin 106137 -> 124423 bytes docs/_build/html/_images/AAV-DJ.png | Bin 0 -> 126548 bytes docs/_build/html/_images/SCH9.png | Bin 0 -> 128889 bytes docs/_build/html/_images/stats.png | Bin 0 -> 48127 bytes docs/_build/html/_sources/pm-doc.rst.txt | 143 ++++++- docs/_build/html/_static/AAV-DJ.png | Bin 0 -> 126548 bytes docs/_build/html/_static/SCH9.png | Bin 0 -> 128889 bytes docs/_build/html/_static/stats.png | Bin 0 -> 48127 bytes docs/_build/html/pm-doc.html | 143 ++++++- docs/_build/html/searchindex.js | 2 +- docs/_static/AAV-DJ.png | Bin 0 -> 126548 bytes docs/_static/SCH9.png | Bin 0 -> 128889 bytes docs/_static/stats.png | Bin 0 -> 48127 bytes docs/pm-doc.rst | 143 ++++++- test-aln.txt | 58 +++ test-def.txt | 152 +++++++ test-par.html | 470 ++++++++++++++++++++++ test-par.txt | 492 +++++++++++++++++++++++ test-stats.txt | 17 + 20 files changed, 1615 insertions(+), 5 deletions(-) create mode 100644 docs/_build/html/_images/AAV-DJ.png create mode 100644 docs/_build/html/_images/SCH9.png create mode 100644 docs/_build/html/_images/stats.png create mode 100644 docs/_build/html/_static/AAV-DJ.png create mode 100644 docs/_build/html/_static/SCH9.png create mode 100644 docs/_build/html/_static/stats.png create mode 100644 docs/_static/AAV-DJ.png create mode 100644 docs/_static/SCH9.png create mode 100644 docs/_static/stats.png create mode 100644 test-aln.txt create mode 100644 test-def.txt create mode 100644 test-par.html create mode 100644 test-par.txt create mode 100644 test-stats.txt diff --git a/docs/_build/doctrees/environment.pickle b/docs/_build/doctrees/environment.pickle index 5b51e8c88b3d7fead4decec534c9ff8329d6d50c..2ce6abaa320fce5275705b00240c25ea2ec0d9a3 100644 GIT binary patch delta 4463 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a/docs/_build/html/_sources/pm-doc.rst.txt +++ b/docs/_build/html/_sources/pm-doc.rst.txt @@ -91,12 +91,16 @@ Note that the prefix 'test' entered with the -o (output) argument became the pre Parent-map can also bypass the sequence analysis and just colorize an existing -par.txt file into a -par.html file. This is useful when the user is not satisfied with the default colors and wants to try different color schemes. In this case, the -C argument is used followed by the name of the -par.txt file. Also, the -p argument must be used followed by a list of parent color pairs. Example:: - python -m parent-map -d -C test-par.txt -p 'AAV1 lavender, AAV2 cyan, AAV3B gold, AAV5 crimson, AAV8 lime, AAV9 coral, AAV6 tan' + python -m parent-map -d -C test-par.txt -p 'AAV2 turquoise, AAV3B tomato, AAV5 lavender, AAV8 gold, AAV9 crimson, AAV6 lime' The following output will be generated, while the newly created html file is automatically displayed (thanks to option -d) in the default web browser:: Colorized version of parental map file saved into file: test-par.html +A portion of the generated html file is shown here: + +.. image:: _static/SCH9.png + Arguments ********* @@ -199,11 +203,58 @@ The -stats.txt file shows a summary of the variant sequences main features, in t #. **Other**: Number of sequence positions that could not be matched to any parent and could not be considered as a variation of one particular parent. +Example of a -stats.txt file: + +.. image:: _static/stats.png Parental mapping **************** In the -par.txt file, each variant complete sequence is displayed along its contributing parental fragments. The parental mapping is simplified as much as possible, so that the variant is completely described using as few parents as possible and as few fragments as possible. Only the necessary parents are shown. If a fragment can be mapped equally to more than one parent, the parent that has the highest overall coverage is chosen. Identities are displayed in the parent lines using an identity symbol ('.' by default). If a mismatch occurs between 2 fragments mapped to the same parents or at a sequence end, it is considered a variation of the parent next to it and displayed as the parent sequence (substitution) or using the gap symbol ('-' by default) in the parent line for insertions or in the variant line for deletions. The -par.txt file is most useful for variant sequences selected from shuffled libraries. Two files are generated: a black and white text file (name ending with -par.txt) and a colorized html version of the same file (name ending with -par.html). In the html file, each parent name and its corresponding identity fragments are colorized using a different color (whenever possible). The default color scheme uses 12 different colors. If the number of parents in the file is greater than 12, the same set of 12 colors is used on the 12 next parents and so on. If the user wishes to apply specific colors, the :ref:`-p argument ` needs to be used followed by a list of parent color pairs, either at the time of the sequence analysis or later with the -C argument to specify the -par.txt file to be colorized again. +Example of a -par.txt file (showing AAV-DJ only):: + + Parental composition of sequence AAV-DJ + + 10 20 30 40 50 60 70 80 90 100 110 + AAV-DJ MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSF + AAV2 .............................................................................................................. + AAV8 + AAV9 ......................... + + 120 130 140 150 160 170 180 190 200 210 220 + AAV-DJ GGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPIGEPPAAPSGVGSLTMAAGGGAPMADNNEGADG + AAV2 .............. ................................................... ............. + AAV8 ................... + AAV9 ..................................... ........................ + + 230 240 250 260 270 280 290 300 310 320 330 + AAV-DJ VGNSSGNWHCDSTWMGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEG + AAV2 ......................................... + AAV8 .. ......................................................... + AAV9 .............................................................................. + + 340 350 360 370 380 390 400 410 420 430 440 + AAV-DJ TKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFQFTYTFEDVPFHSSYAHSQSLDRLMNPLI + AAV2 + AAV8 .............................................................................................................. + + 450 460 470 480 490 500 510 520 530 540 550 + AAV-DJ DQYLYYLSRTQTTGGTTNTQTLGFSQGGPNTMANQAKNWLPGPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSE + AAV2 ......................................................................... + AAV8 ................A.................................. + + 560 570 580 590 600 610 620 630 640 650 660 + AAV-DJ KTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQGVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPADPP + AAV2 ........................................................................................................... + AAV8 ............................... + + 670 680 690 700 710 720 730 + AAV-DJ TTFNQSKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTSVDFAVNTEGVYSEPRPIGTRYLTRNL + AAV8 ............................................................................. + +Same map from the corresponding -par.html file, using default colors: + +.. image:: _static/AAV-DJ.png Sequence definitions ******************** @@ -220,10 +271,100 @@ The -def.txt file shows comprehensive definitions of variant sequences in terms #. **Parent sequence**: In the case of substitutions or deletions, the corresponding parental sequence is displayed here. +Here is an example of a -def.txt file:: + + Variant name: OLIG001 + Variant region Parent/feature Parent region Variant sequence Parent sequence`` + 1-124 AAV2 1-124 + 86-156 AAV9 86-156 + 136-178 AAV6 136-178 + 165-262 AAV9 165-262 + 234-341 AAV8 235-342 + 333-373 AAV2 331-371 + 343-531 AAV8 344-532 + 532 substitution 533 K E + 533-725 AAV8 534-726 + 726 unmatched H + 727-737 AAV6 726-736 + + Variant name: AAV2i8 + Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-584 AAV2 1-584 + 582-590 AAV8 585-593 + 591-735 AAV2 591-735 + + Variant name: rAAV2-retro + Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-381 AAV2 1-381 + 382 substitution 382 D N + 383-587 AAV2 383-587 + 588-597 insertion 587/588 LADQDYTKTA + 598-717 AAV2 588-707 + 718 substitution 708 I V + 719-745 AAV2 709-735`` Alignments ********** The -aln.txt file consists of two parts: the first concerns sequences derived from a single parent, while the second part is about sequences derived from multiple parents. In each part, variants derived from the same main parents are aligned together against their main parent. Only regions that differ from the parent in any of the variants are shown, and the region position is indicated by a number (numbering is the one defined in the parental sequence file as explained in :ref:`parents `). Sequence identity is shown using the identity symbol (default: '.'), gaps are shown using the gap symbol (default: '-'). Insertions are indicated by a gap in the parent sequence, deletions by a gap in the variant sequence. For substitutions, the actual sequence is shown. Increasing the region area (using the -e argument) allows to show more sequence context and to combine neighboring regions. The -aln.txt file is most useful for variants derived from a single parent (such as from insertion or targeted substitution libraries), as it allows to compare variants in discrete regions, which can be much larger in number and size when variants are derived from multiple parents (as in shuffled libraries). +Example of -aln.txt file:: + + Alignment of variant sequences against their main parental sequence - single parent + + 239 251 262 271 381 443 449 458 491 498 506 587 699 703 707 729 + AAV2 VIT TYN SQS HYF NNG YYL NTPSGTT SRLQF TSADN SEYSWTG TKY N----------R QYT NYN SVN RYL + LI-A ... ... .A. ... ... .F. .SEGSL. .S.G. .DGE. .DF.... ... .----------. ... ... ... ... + LI-C ... ... .A. ... ... ... ....... ..... ..... ..F..P. .T. .----------. ... ... ... ... + AAV2-sept-Y-F ... .F. ... .F. ... .F. ....... ..... ..... ..F.... ... .----------. .F. .F. ... .F. + 7m8 .T. ... ... ... ... ... ....... ..... ..... ....... ... .LALGETTRPA. ... ... .I. ... + rAAV2-retro ... ... ... ... .D. ... ....... ..... ..... ....... ... .LADQDYTKTA. ... ... .I. ... + + + 588 + AAV9 Q-------A + AAV-F .FVVGQSY. + AAV-PHP.B .TLAVPFK. + + + + Alignment of variant sequences against their main parental sequence - multiple parents + + + 124 134 161 167 178 187 197 204 232 261 270 309 326 339 371 409 448 455 + AAV2 RVL EPVK KAG ARK DADS PLGQP GLGTNT ATGSG STWMG S-SQS-GAS NHY KRLNF NDGTTT TVQ MVP FTFSY TNTPSG TTQSRLQF + AAV2i8 ... .... ... ... .... ..... ...... ..... ..... .-...-... ... ..... ...... ... ... ..... ...... ........ + AAV-DJ .L. .AA. ... ... .... .I.E. .V.SL. .A.G. ..... .N.T.G.S. .A. ...S. .E..K. .I. .I. .Q.T. .Q.TG. .NTQT.G. + NP84 ... .... .T. .K. .SE. ...E. ...... ..... .Q.L. .-...-... ... .K.S. ...... ... ... ..... ...... ........ + + 464 531 584 655 661 704 712 + AAV2 QAGASDIRDQSRN EKF QRGNRQAA ANPST FSAAKFAS YNKSVNV FTVDTNG + AAV2i8 ............. ... .QQ.TAP. ..... ........ ....... ....... + AAV-DJ .G.PNTMAN.AK. ... ........ .D.P. .NQS.LN. .Y..TS. .A.N.E. + NP84 ............. .E. .G...... ..... ........ ....... ....... + + + 1 12 29 33 49 54 59 65 75 101 114 580 + AAV5 MSFVDHPP E-EVGEGLREFLGLEAG PKP PNQQHQDQAR YNY PGN DRG NRADEVARE DISYNEQLEAG EKLADD GKA PAT + AAV2.5T .AADGYL. .DTLS..I.QWWK.KP. .P. .AER.K.DS. .K. .F. .K. .E..AA.L. .KA.DR..DS. .R.KE. .R. .T. + + + 13 20 28 66 80 91 104 124 134 147 151 157 162 168 179 188 200 205 211 233 342 532 726 + AAV8 DNL REWWAL GAPKPKANQQKQDDGR NAA DQQLQAG LRY LQE RVL EGA EPS QRSP STG KKG ARK DSE PLG GPNT AAG PMA STW TIQ DEE PRP + OLIG001 .T. .Q..K. .P.P..PAERHK..S. .E. .R..DS. .K. .K. .L. .A. .Q. .-E. .S. .T. .K. .T. .I. .SL. .S. .V. .Q. .V. .K. .H. + + 736 + AAV8 RNL + OLIG001 .P. + + + 23 30 37 55 91 104 124 313 326 341 415 448 482 490 + AAV9 WAL PQP QHQDNAR PGN LKY LKE RLL LNF TDNNGVK TVQ YEFENV SKTINGSG-QNQQTLKFSVAGPSNMAVQGRNYIP PSY STTVTQN + SCH2 .D. .K. .K..DG. .F. .R. .Q. .V. .S. .Q.E.T. .I. .T..D. .R.NTP..TTT.SR.Q..Q..A.DIRD.S..WL. .C. .K.SAD. + SCH9 .D. .K. .K..DG. .F. .R. .Q. .V. .S. .Q.E.T. .I. ...... 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zF*&SS_)hKa68p6OjkhW{Ay=(k3A>))0ej7-e2M-R%v6Jv6&LuD2%@-#Vs=!Gv6*?{ zLytDFy|8P!1@(|WN?A*-GG{|n-BS!wLIQR?%%L_qeb|9A6RaS<%bm=V@tlcK55^OQ z=s9__`7=`BZUE4Br~l0uZoVNAJ$BldzPi`=2hLgb8+O#nt`qKI!gqwW>u{*K*qC

Usage

Note that the prefix ‘test’ entered with the -o (output) argument became the prefix of the 4 output files. Also note that the sequence name change (first line) can be avoided by using the -s argument (maximum name size) followed by the value 13 (the sequence name ‘AAV2-sept-Y-F’ is 13 character long, while the default value for -s is 12).

Parent-map can also bypass the sequence analysis and just colorize an existing -par.txt file into a -par.html file. This is useful when the user is not satisfied with the default colors and wants to try different color schemes. In this case, the -C argument is used followed by the name of the -par.txt file. Also, the -p argument must be used followed by a list of parent color pairs. Example:

-
python -m parent-map -d -C test-par.txt -p 'AAV1 lavender, AAV2 cyan, AAV3B gold, AAV5 crimson, AAV8 lime, AAV9 coral, AAV6 tan'
+
python -m parent-map -d -C test-par.txt -p 'AAV2 turquoise, AAV3B tomato, AAV5 lavender, AAV8 gold, AAV9 crimson, AAV6 lime'
 

The following output will be generated, while the newly created html file is automatically displayed (thanks to option -d) in the default web browser:

Colorized version of parental map file saved into file: test-par.html
 
+

A portion of the generated html file is shown here:

+_images/SCH9.png

Arguments¶

The same parameters are available in both modes (graphic and command-line) but some default values might differ.

@@ -301,10 +303,55 @@

Statistics

Parental mapping¶

In the -par.txt file, each variant complete sequence is displayed along its contributing parental fragments. The parental mapping is simplified as much as possible, so that the variant is completely described using as few parents as possible and as few fragments as possible. Only the necessary parents are shown. If a fragment can be mapped equally to more than one parent, the parent that has the highest overall coverage is chosen. Identities are displayed in the parent lines using an identity symbol (‘.’ by default). If a mismatch occurs between 2 fragments mapped to the same parents or at a sequence end, it is considered a variation of the parent next to it and displayed as the parent sequence (substitution) or using the gap symbol (‘-‘ by default) in the parent line for insertions or in the variant line for deletions. The -par.txt file is most useful for variant sequences selected from shuffled libraries. Two files are generated: a black and white text file (name ending with -par.txt) and a colorized html version of the same file (name ending with -par.html). In the html file, each parent name and its corresponding identity fragments are colorized using a different color (whenever possible). The default color scheme uses 12 different colors. If the number of parents in the file is greater than 12, the same set of 12 colors is used on the 12 next parents and so on. If the user wishes to apply specific colors, the -p argument needs to be used followed by a list of parent color pairs, either at the time of the sequence analysis or later with the -C argument to specify the -par.txt file to be colorized again.

+

Example of a -par.txt file (showing AAV-DJ only):

+
Parental composition of sequence AAV-DJ
+
+                      10        20        30        40        50        60        70        80        90       100       110
+AAV-DJ        MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSF
+AAV2          ..............................................................................................................
+AAV8
+AAV9                                                                                               .........................
+
+                     120       130       140       150       160       170       180       190       200       210       220
+AAV-DJ        GGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPIGEPPAAPSGVGSLTMAAGGGAPMADNNEGADG
+AAV2          ..............            ...................................................                    .............
+AAV8                                                                                                     ...................
+AAV9          .....................................                                 ........................
+
+                     230       240       250       260       270       280       290       300       310       320       330
+AAV-DJ        VGNSSGNWHCDSTWMGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEG
+AAV2          .........................................
+AAV8          ..                                                   .........................................................
+AAV9                         ..............................................................................
+
+                     340       350       360       370       380       390       400       410       420       430       440
+AAV-DJ        TKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFQFTYTFEDVPFHSSYAHSQSLDRLMNPLI
+AAV2
+AAV8          ..............................................................................................................
+
+                     450       460       470       480       490       500       510       520       530       540       550
+AAV-DJ        DQYLYYLSRTQTTGGTTNTQTLGFSQGGPNTMANQAKNWLPGPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSE
+AAV2                                               .........................................................................
+AAV8          ................A..................................
+
+                     560       570       580       590       600       610       620       630       640       650       660
+AAV-DJ        KTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQGVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPADPP
+AAV2          ...........................................................................................................
+AAV8                                                                                         ...............................
+
+                     670       680       690       700       710       720       730
+AAV-DJ        TTFNQSKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTSVDFAVNTEGVYSEPRPIGTRYLTRNL
+AAV8          .............................................................................
+
+
+

Same map from the corresponding -par.html file, using default colors:

+_images/AAV-DJ.png

Alignments¶

-

The -aln.txt file consists of two parts: the first concerns sequences derived from a single parent, while the second part is about sequences derived from multiple parents. In each part, variants derived from the same main parents are aligned together against their main parent. Only regions that differ from the parent in any of the variants are shown, and the region position is indicated by a number (numbering is the one defined in the parental sequence file as explained in parents). Sequence identity is shown using the identity symbol (default: ‘.’), gaps are shown using the gap symbol (default: ‘-‘). Insertions are indicated by a gap in the parent sequence, deletions by a gap in the variant sequence. For substitutions, the actual sequence is shown. Increasing the region area (using the -e argument) allows to show more sequence context and to combine neighboring regions. The -aln.txt file is most useful for variants derived from a single parent (such as from insertion or targeted substitution libraries), as it allows to compare variants in discrete regions, which can be much larger in number and size when variants are derived from multiple parents (as in shuffled libraries).

+

The -aln.txt file consists of two parts: the first concerns sequences derived from a single parent, while the second part is about sequences derived from multiple parents. In each part, variants derived from the same main parents are aligned together against their main parent. Only regions that differ from the parent in any of the variants are shown, and the region position is indicated by a number (numbering is the one defined in the parental sequence file as explained in parents). Sequence identity is shown using the identity symbol (default: ‘.’), gaps are shown using the gap symbol (default: ‘-‘). Insertions are indicated by a gap in the parent sequence, deletions by a gap in the variant sequence. For substitutions, the actual sequence is shown. Increasing the region area (using the -e argument) allows to show more sequence context and to combine neighboring regions. The -aln.txt file is most useful for variants derived from a single parent (such as from insertion or targeted substitution libraries), as it allows to compare variants in discrete regions, which can be much larger in number and size when variants are derived from multiple parents (as in shuffled libraries). +Example of -aln.txt file:

+
Alignment of variant sequences against their main parental sequence - single parent
+
+              239 251 262 271 381 443 449     458   491   498     506 587          699 703 707 729
+AAV2          VIT TYN SQS HYF NNG YYL NTPSGTT SRLQF TSADN SEYSWTG TKY N----------R QYT NYN SVN RYL
+LI-A          ... ... .A. ... ... .F. .SEGSL. .S.G. .DGE. .DF.... ... .----------. ... ... ... ...
+LI-C          ... ... .A. ... ... ... ....... ..... ..... ..F..P. .T. .----------. ... ... ... ...
+AAV2-sept-Y-F ... .F. ... .F. ... .F. ....... ..... ..... ..F.... ... .----------. .F. .F. ... .F.
+7m8           .T. ... ... ... ... ... ....... ..... ..... ....... ... .LALGETTRPA. ... ... .I. ...
+rAAV2-retro   ... ... ... ... .D. ... ....... ..... ..... ....... ... .LADQDYTKTA. ... ... .I. ...
+
+
+              588
+AAV9          Q-------A
+AAV-F         .FVVGQSY.
+AAV-PHP.B     .TLAVPFK.
+
+
+
+Alignment of variant sequences against their main parental sequence - multiple parents
+
+
+              124 134  161 167 178  187   197    204   232   261       270 309   326    339 371 409   448    455
+AAV2          RVL EPVK KAG ARK DADS PLGQP GLGTNT ATGSG STWMG S-SQS-GAS NHY KRLNF NDGTTT TVQ MVP FTFSY TNTPSG TTQSRLQF
+AAV2i8        ... .... ... ... .... ..... ...... ..... ..... .-...-... ... ..... ...... ... ... ..... ...... ........
+AAV-DJ        .L. .AA. ... ... .... .I.E. .V.SL. .A.G. ..... .N.T.G.S. .A. ...S. .E..K. .I. .I. .Q.T. .Q.TG. .NTQT.G.
+NP84          ... .... .T. .K. .SE. ...E. ...... ..... .Q.L. .-...-... ... .K.S. ...... ... ... ..... ...... ........
+
+              464           531 584      655   661      704     712
+AAV2          QAGASDIRDQSRN EKF QRGNRQAA ANPST FSAAKFAS YNKSVNV FTVDTNG
+AAV2i8        ............. ... .QQ.TAP. ..... ........ ....... .......
+AAV-DJ        .G.PNTMAN.AK. ... ........ .D.P. .NQS.LN. .Y..TS. .A.N.E.
+NP84          ............. .E. .G...... ..... ........ ....... .......
+
+
+              1        12                29  33         49  54  59  65        75          101    114 580
+AAV5          MSFVDHPP E-EVGEGLREFLGLEAG PKP PNQQHQDQAR YNY PGN DRG NRADEVARE DISYNEQLEAG EKLADD GKA PAT
+AAV2.5T       .AADGYL. .DTLS..I.QWWK.KP. .P. .AER.K.DS. .K. .F. .K. .E..AA.L. .KA.DR..DS. .R.KE. .R. .T.
+
+
+              13  20     28               66  80      91  104 124 134 147 151  157 162 168 179 188 200  205 211 233 342 532 726
+AAV8          DNL REWWAL GAPKPKANQQKQDDGR NAA DQQLQAG LRY LQE RVL EGA EPS QRSP STG KKG ARK DSE PLG GPNT AAG PMA STW TIQ DEE PRP
+OLIG001       .T. .Q..K. .P.P..PAERHK..S. .E. .R..DS. .K. .K. .L. .A. .Q. .-E. .S. .T. .K. .T. .I. .SL. .S. .V. .Q. .V. .K. .H.
+
+              736
+AAV8          RNL
+OLIG001       .P.
+
+
+              23  30  37      55  91  104 124 313 326     341 415    448                                482 490
+AAV9          WAL PQP QHQDNAR PGN LKY LKE RLL LNF TDNNGVK TVQ YEFENV SKTINGSG-QNQQTLKFSVAGPSNMAVQGRNYIP PSY STTVTQN
+SCH2          .D. .K. .K..DG. .F. .R. .Q. .V. .S. .Q.E.T. .I. .T..D. .R.NTP..TTT.SR.Q..Q..A.DIRD.S..WL. .C. .K.SAD.
+SCH9          .D. .K. .K..DG. .F. .R. .Q. .V. .S. .Q.E.T. .I. ...... ........-......................WL. .C. .K.SAD.
+
+              500          514    528     536    547    554  561 566 575
+AAV9          EFAWPGASSWAL RNSLMN KEGEDRF PLSGSL GTGRDN DADK TNE IKT ESYGQVATNHQSAQAQAQTGWVQNQGIL
+SCH2          .YS.T..TKYH. .D..V. .DD.EK. .Q..V. .SEKT. .IE. .D. .R. .Q..S.S..L.RGNR..A.AD.NT..V.
+SCH9          .YS.T..TKYH. .D..V. .DD.EK. .Q..V. .SEKT. .IE. .D. .R. .Q..S.S..L.RGNR..A.AD.NT..V.
+
+

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that the prefix 'test' entered with the -o (output) argument became the pre Parent-map can also bypass the sequence analysis and just colorize an existing -par.txt file into a -par.html file. This is useful when the user is not satisfied with the default colors and wants to try different color schemes. In this case, the -C argument is used followed by the name of the -par.txt file. Also, the -p argument must be used followed by a list of parent color pairs. Example:: - python -m parent-map -d -C test-par.txt -p 'AAV1 lavender, AAV2 cyan, AAV3B gold, AAV5 crimson, AAV8 lime, AAV9 coral, AAV6 tan' + python -m parent-map -d -C test-par.txt -p 'AAV2 turquoise, AAV3B tomato, AAV5 lavender, AAV8 gold, AAV9 crimson, AAV6 lime' The following output will be generated, while the newly created html file is automatically displayed (thanks to option -d) in the default web browser:: Colorized version of parental map file saved into file: test-par.html +A portion of the generated html file is shown here: + +.. image:: _static/SCH9.png + Arguments ********* @@ -199,11 +203,58 @@ The -stats.txt file shows a summary of the variant sequences main features, in t #. **Other**: Number of sequence positions that could not be matched to any parent and could not be considered as a variation of one particular parent. +Example of a -stats.txt file: + +.. image:: _static/stats.png Parental mapping **************** In the -par.txt file, each variant complete sequence is displayed along its contributing parental fragments. The parental mapping is simplified as much as possible, so that the variant is completely described using as few parents as possible and as few fragments as possible. Only the necessary parents are shown. If a fragment can be mapped equally to more than one parent, the parent that has the highest overall coverage is chosen. Identities are displayed in the parent lines using an identity symbol ('.' by default). If a mismatch occurs between 2 fragments mapped to the same parents or at a sequence end, it is considered a variation of the parent next to it and displayed as the parent sequence (substitution) or using the gap symbol ('-' by default) in the parent line for insertions or in the variant line for deletions. The -par.txt file is most useful for variant sequences selected from shuffled libraries. Two files are generated: a black and white text file (name ending with -par.txt) and a colorized html version of the same file (name ending with -par.html). In the html file, each parent name and its corresponding identity fragments are colorized using a different color (whenever possible). The default color scheme uses 12 different colors. If the number of parents in the file is greater than 12, the same set of 12 colors is used on the 12 next parents and so on. If the user wishes to apply specific colors, the :ref:`-p argument ` needs to be used followed by a list of parent color pairs, either at the time of the sequence analysis or later with the -C argument to specify the -par.txt file to be colorized again. +Example of a -par.txt file (showing AAV-DJ only):: + + Parental composition of sequence AAV-DJ + + 10 20 30 40 50 60 70 80 90 100 110 + AAV-DJ MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSF + AAV2 .............................................................................................................. + AAV8 + AAV9 ......................... + + 120 130 140 150 160 170 180 190 200 210 220 + AAV-DJ GGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPIGEPPAAPSGVGSLTMAAGGGAPMADNNEGADG + AAV2 .............. ................................................... ............. + AAV8 ................... + AAV9 ..................................... ........................ + + 230 240 250 260 270 280 290 300 310 320 330 + AAV-DJ VGNSSGNWHCDSTWMGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEG + AAV2 ......................................... + AAV8 .. ......................................................... + AAV9 .............................................................................. + + 340 350 360 370 380 390 400 410 420 430 440 + AAV-DJ TKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFQFTYTFEDVPFHSSYAHSQSLDRLMNPLI + AAV2 + AAV8 .............................................................................................................. + + 450 460 470 480 490 500 510 520 530 540 550 + AAV-DJ DQYLYYLSRTQTTGGTTNTQTLGFSQGGPNTMANQAKNWLPGPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSE + AAV2 ......................................................................... + AAV8 ................A.................................. + + 560 570 580 590 600 610 620 630 640 650 660 + AAV-DJ KTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQGVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPADPP + AAV2 ........................................................................................................... + AAV8 ............................... + + 670 680 690 700 710 720 730 + AAV-DJ TTFNQSKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTSVDFAVNTEGVYSEPRPIGTRYLTRNL + AAV8 ............................................................................. + +Same map from the corresponding -par.html file, using default colors: + +.. image:: _static/AAV-DJ.png Sequence definitions ******************** @@ -220,10 +271,100 @@ The -def.txt file shows comprehensive definitions of variant sequences in terms #. **Parent sequence**: In the case of substitutions or deletions, the corresponding parental sequence is displayed here. +Here is an example of a -def.txt file:: + + Variant name: OLIG001 + Variant region Parent/feature Parent region Variant sequence Parent sequence`` + 1-124 AAV2 1-124 + 86-156 AAV9 86-156 + 136-178 AAV6 136-178 + 165-262 AAV9 165-262 + 234-341 AAV8 235-342 + 333-373 AAV2 331-371 + 343-531 AAV8 344-532 + 532 substitution 533 K E + 533-725 AAV8 534-726 + 726 unmatched H + 727-737 AAV6 726-736 + + Variant name: AAV2i8 + Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-584 AAV2 1-584 + 582-590 AAV8 585-593 + 591-735 AAV2 591-735 + + Variant name: rAAV2-retro + Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-381 AAV2 1-381 + 382 substitution 382 D N + 383-587 AAV2 383-587 + 588-597 insertion 587/588 LADQDYTKTA + 598-717 AAV2 588-707 + 718 substitution 708 I V + 719-745 AAV2 709-735`` Alignments ********** The -aln.txt file consists of two parts: the first concerns sequences derived from a single parent, while the second part is about sequences derived from multiple parents. In each part, variants derived from the same main parents are aligned together against their main parent. Only regions that differ from the parent in any of the variants are shown, and the region position is indicated by a number (numbering is the one defined in the parental sequence file as explained in :ref:`parents `). Sequence identity is shown using the identity symbol (default: '.'), gaps are shown using the gap symbol (default: '-'). Insertions are indicated by a gap in the parent sequence, deletions by a gap in the variant sequence. For substitutions, the actual sequence is shown. Increasing the region area (using the -e argument) allows to show more sequence context and to combine neighboring regions. The -aln.txt file is most useful for variants derived from a single parent (such as from insertion or targeted substitution libraries), as it allows to compare variants in discrete regions, which can be much larger in number and size when variants are derived from multiple parents (as in shuffled libraries). +Example of -aln.txt file:: + + Alignment of variant sequences against their main parental sequence - single parent + + 239 251 262 271 381 443 449 458 491 498 506 587 699 703 707 729 + AAV2 VIT TYN SQS HYF NNG YYL NTPSGTT SRLQF TSADN SEYSWTG TKY N----------R QYT NYN SVN RYL + LI-A ... ... .A. ... ... .F. .SEGSL. .S.G. .DGE. .DF.... ... .----------. ... ... ... ... + LI-C ... ... .A. ... ... ... ....... ..... ..... ..F..P. .T. .----------. ... ... ... ... + AAV2-sept-Y-F ... .F. ... .F. ... .F. ....... ..... ..... ..F.... ... .----------. .F. .F. ... .F. + 7m8 .T. ... ... ... ... ... ....... ..... ..... ....... ... .LALGETTRPA. ... ... .I. ... + rAAV2-retro ... ... ... ... .D. ... ....... ..... ..... ....... ... .LADQDYTKTA. ... ... .I. ... + + + 588 + AAV9 Q-------A + AAV-F .FVVGQSY. + AAV-PHP.B .TLAVPFK. + + + + Alignment of variant sequences against their main parental sequence - multiple parents + + + 124 134 161 167 178 187 197 204 232 261 270 309 326 339 371 409 448 455 + AAV2 RVL EPVK KAG ARK DADS PLGQP GLGTNT ATGSG STWMG S-SQS-GAS NHY KRLNF NDGTTT TVQ MVP FTFSY TNTPSG TTQSRLQF + AAV2i8 ... .... ... ... .... ..... ...... ..... ..... .-...-... ... ..... ...... ... ... ..... ...... ........ + AAV-DJ .L. .AA. ... ... .... .I.E. .V.SL. .A.G. ..... .N.T.G.S. .A. ...S. .E..K. .I. .I. .Q.T. .Q.TG. .NTQT.G. + NP84 ... .... .T. .K. .SE. ...E. ...... ..... .Q.L. .-...-... ... .K.S. ...... ... ... ..... ...... ........ + + 464 531 584 655 661 704 712 + AAV2 QAGASDIRDQSRN EKF QRGNRQAA ANPST FSAAKFAS YNKSVNV FTVDTNG + AAV2i8 ............. ... .QQ.TAP. ..... ........ ....... ....... + AAV-DJ .G.PNTMAN.AK. ... ........ .D.P. .NQS.LN. .Y..TS. .A.N.E. + NP84 ............. .E. .G...... ..... ........ ....... ....... + + + 1 12 29 33 49 54 59 65 75 101 114 580 + AAV5 MSFVDHPP E-EVGEGLREFLGLEAG PKP PNQQHQDQAR YNY PGN DRG NRADEVARE DISYNEQLEAG EKLADD GKA PAT + AAV2.5T .AADGYL. .DTLS..I.QWWK.KP. .P. .AER.K.DS. .K. .F. .K. .E..AA.L. .KA.DR..DS. .R.KE. .R. .T. + + + 13 20 28 66 80 91 104 124 134 147 151 157 162 168 179 188 200 205 211 233 342 532 726 + AAV8 DNL REWWAL GAPKPKANQQKQDDGR NAA DQQLQAG LRY LQE RVL EGA EPS QRSP STG KKG ARK DSE PLG GPNT AAG PMA STW TIQ DEE PRP + OLIG001 .T. .Q..K. .P.P..PAERHK..S. .E. .R..DS. .K. .K. .L. .A. .Q. .-E. .S. .T. .K. .T. .I. .SL. .S. .V. .Q. .V. .K. .H. + + 736 + AAV8 RNL + OLIG001 .P. + + + 23 30 37 55 91 104 124 313 326 341 415 448 482 490 + AAV9 WAL PQP QHQDNAR PGN LKY LKE RLL LNF TDNNGVK TVQ YEFENV SKTINGSG-QNQQTLKFSVAGPSNMAVQGRNYIP PSY STTVTQN + SCH2 .D. .K. .K..DG. .F. .R. .Q. .V. .S. .Q.E.T. .I. .T..D. .R.NTP..TTT.SR.Q..Q..A.DIRD.S..WL. .C. .K.SAD. + SCH9 .D. .K. .K..DG. .F. .R. .Q. .V. .S. .Q.E.T. .I. ...... ........-......................WL. .C. .K.SAD. + + 500 514 528 536 547 554 561 566 575 + AAV9 EFAWPGASSWAL RNSLMN KEGEDRF PLSGSL GTGRDN DADK TNE IKT ESYGQVATNHQSAQAQAQTGWVQNQGIL + SCH2 .YS.T..TKYH. .D..V. .DD.EK. .Q..V. .SEKT. .IE. .D. .R. .Q..S.S..L.RGNR..A.AD.NT..V. + SCH9 .YS.T..TKYH. .D..V. .DD.EK. .Q..V. .SEKT. .IE. .D. .R. .Q..S.S..L.RGNR..A.AD.NT..V. | Examples diff --git a/test-aln.txt b/test-aln.txt new file mode 100644 index 0000000..a08c03f --- /dev/null +++ b/test-aln.txt @@ -0,0 +1,58 @@ + +Alignment of variant sequences against their main parental sequence - single parent + + + 239 251 262 271 381 443 449 458 491 498 506 587 699 703 707 729 +AAV2 VIT TYN SQS HYF NNG YYL NTPSGTT SRLQF TSADN SEYSWTG TKY N----------R QYT NYN SVN RYL +LI-A ... ... .A. ... ... .F. .SEGSL. .S.G. .DGE. .DF.... ... .----------. ... ... ... ... +LI-C ... ... .A. ... ... ... ....... ..... ..... ..F..P. .T. .----------. ... ... ... ... +S14 ... .F. ... .F. ... .F. ....... ..... ..... ..F.... ... .----------. .F. .F. ... .F. +7m8 .T. ... ... ... ... ... ....... ..... ..... ....... ... .LALGETTRPA. ... ... .I. ... +rAAV2-retro ... ... ... ... .D. ... ....... ..... ..... ....... ... .LADQDYTKTA. ... ... .I. ... + + + 588 +AAV9 Q-------A +AAV-F .FVVGQSY. +AAV-PHP.B .TLAVPFK. + + + +Alignment of variant sequences against their main parental sequence - multiple parents + + + 124 134 161 167 178 187 197 204 232 261 270 309 326 339 371 409 448 455 464 531 +AAV2 RVL EPVK KAG ARK DADS PLGQP GLGTNT ATGSG STWMG S-SQS-GAS NHY KRLNF NDGTTT TVQ MVP FTFSY TNTPSG TTQSRLQF QAGASDIRDQSRN EKF +AAV2i8 ... .... ... ... .... ..... ...... ..... ..... .-...-... ... ..... ...... ... ... ..... ...... ........ ............. ... +AAV-DJ .L. .AA. ... ... .... .I.E. .V.SL. .A.G. ..... .N.T.G.S. .A. ...S. .E..K. .I. .I. .Q.T. .Q.TG. .NTQT.G. .G.PNTMAN.AK. ... +NP84 ... .... .T. .K. .SE. ...E. ...... ..... .Q.L. .-...-... ... .K.S. ...... ... ... ..... ...... ........ ............. .E. + + 584 655 661 704 712 +AAV2 QRGNRQAA ANPST FSAAKFAS YNKSVNV FTVDTNG +AAV2i8 .QQ.TAP. ..... ........ ....... ....... +AAV-DJ ........ .D.P. .NQS.LN. .Y..TS. .A.N.E. +NP84 .G...... ..... ........ ....... ....... + + + 1 12 29 33 49 54 59 65 75 101 114 580 +AAV5 MSFVDHPP E-EVGEGLREFLGLEAG PKP PNQQHQDQAR YNY PGN DRG NRADEVARE DISYNEQLEAG EKLADD GKA PAT +AAV2.5T .AADGYL. .DTLS..I.QWWK.KP. .P. .AER.K.DS. .K. .F. .K. .E..AA.L. .KA.DR..DS. .R.KE. .R. .T. + + + 13 20 28 66 80 91 104 124 134 147 151 157 162 168 179 188 200 205 211 233 342 532 726 736 +AAV8 DNL REWWAL GAPKPKANQQKQDDGR NAA DQQLQAG LRY LQE RVL EGA EPS QRSP STG KKG ARK DSE PLG GPNT AAG PMA STW TIQ DEE PRP RNL +OLIG001 .T. .Q..K. .P.P..PAERHK..S. .E. .R..DS. .K. .K. .L. .A. .Q. .-E. .S. .T. .K. .T. .I. .SL. .S. .V. .Q. .V. .K. .H. .P. + + + 23 30 37 55 91 104 124 313 326 341 415 448 482 490 500 514 +AAV9 WAL PQP QHQDNAR PGN LKY LKE RLL LNF TDNNGVK TVQ YEFENV SKTINGSG-QNQQTLKFSVAGPSNMAVQGRNYIP PSY STTVTQN EFAWPGASSWAL RNSLMN +SCH2 .D. .K. .K..DG. .F. .R. .Q. .V. .S. .Q.E.T. .I. .T..D. .R.NTP..TTT.SR.Q..Q..A.DIRD.S..WL. .C. .K.SAD. .YS.T..TKYH. .D..V. +SCH9 .D. .K. .K..DG. .F. .R. .Q. .V. .S. .Q.E.T. .I. ...... ........-......................WL. .C. .K.SAD. .YS.T..TKYH. .D..V. + + 528 536 547 554 561 566 575 +AAV9 KEGEDRF PLSGSL GTGRDN DADK TNE IKT ESYGQVATNHQSAQAQAQTGWVQNQGIL +SCH2 .DD.EK. .Q..V. .SEKT. .IE. .D. .R. .Q..S.S..L.RGNR..A.AD.NT..V. +SCH9 .DD.EK. .Q..V. .SEKT. .IE. .D. .R. .Q..S.S..L.RGNR..A.AD.NT..V. + + + diff --git a/test-def.txt b/test-def.txt new file mode 100644 index 0000000..a4fd44b --- /dev/null +++ b/test-def.txt @@ -0,0 +1,152 @@ +Variant name: SCH2 +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-128 AAV6 1-128 + 126-313 AAV9 126-313 + 274-348 AAV8 275-349 + 343-415 AAV9 343-415 + 413-628 AAV2 411-626 + 603-737 AAV9 602-736 + +Variant name: SCH9 +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-128 AAV6 1-128 + 126-313 AAV9 126-313 + 274-348 AAV8 275-349 + 343-477 AAV9 343-477 + 476-627 AAV2 475-626 + 602-736 AAV9 602-736 + +Variant name: AAV-F +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-588 AAV9 1-588 + 589-595 insertion 588/589 FVVGQSY + 596-743 AAV9 589-736 + +Variant name: AAV-DJ +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-124 AAV2 1-124 + 86-147 AAV9 86-147 + 137-187 AAV2 137-187 + 181-204 AAV9 181-204 + 202-222 AAV8 203-223 + 208-261 AAV2 208-261 + 236-313 AAV9 236-313 + 274-456 AAV8 275-457 + 457 substitution 458 T A + 458-491 AAV8 459-492 + 478-657 AAV2 476-655 + 630-737 AAV8 631-738 + +Variant name: AAV-PHP.B +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-588 AAV9 1-588 + 589-595 insertion 588/589 TLAVPFK + 596-743 AAV9 589-736 + +Variant name: AAV2.5T +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-128 AAV2 1-128 + 117-581 AAV5 116-580 + 582 substitution 581 T A + 583-725 AAV5 582-724 + +Variant name: 7m8 +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-239 AAV2 1-239 + 240 substitution 240 T I + 241-587 AAV2 241-587 + 588-597 insertion 587/588 LALGETTRPA + 598-717 AAV2 588-707 + 718 substitution 708 I V + 719-745 AAV2 709-735 + +Variant name: LI-A +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-262 AAV2 1-262 + 263 substitution 263 A Q + 264-443 AAV2 264-443 + 444 substitution 444 F Y + 445-449 AAV2 445-449 + 450-454 substitution 450-454 SEGSL TPSGT + 455-458 AAV2 455-458 + 459 substitution 459 S R + 460 AAV2 460 + 461 substitution 461 G Q + 462-491 AAV2 462-491 + 492-494 substitution 492-494 DGE SAD + 495-498 AAV2 495-498 + 499-500 substitution 499-500 DF EY + 501-735 AAV2 501-735 + +Variant name: LI-C +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-262 AAV2 1-262 + 263 substitution 263 A Q + 264-499 AAV2 264-499 + 500 substitution 500 F Y + 501-502 AAV2 501-502 + 503 substitution 503 P T + 504-506 AAV2 504-506 + 507 substitution 507 T K + 508-735 AAV2 508-735 + +Variant name: NP84 +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-161 AAV2 1-161 + 160-193 AAV6 160-193 + 191-232 AAV2 191-232 + 208-409 AAV3B 208-409 + 313-531 AAV2 313-531 + 532 substitution 532 E K + 533-584 AAV2 533-584 + 585 substitution 585 G R + 586-735 AAV2 586-735 + +Variant name: OLIG001 +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-124 AAV2 1-124 + 86-156 AAV9 86-156 + 136-178 AAV6 136-178 + 165-262 AAV9 165-262 + 234-341 AAV8 235-342 + 333-373 AAV2 331-371 + 343-531 AAV8 344-532 + 532 substitution 533 K E + 533-725 AAV8 534-726 + 726 unmatched H + 727-737 AAV6 726-736 + +Variant name: AAV2i8 +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-584 AAV2 1-584 + 582-590 AAV8 585-593 + 591-735 AAV2 591-735 + +Variant name: rAAV2-retro +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-381 AAV2 1-381 + 382 substitution 382 D N + 383-587 AAV2 383-587 + 588-597 insertion 587/588 LADQDYTKTA + 598-717 AAV2 588-707 + 718 substitution 708 I V + 719-745 AAV2 709-735 + +Variant name: S14 +Variant region Parent/feature Parent region Variant sequence Parent sequence + 1-251 AAV2 1-251 + 252 substitution 252 F Y + 253-271 AAV2 253-271 + 272 substitution 272 F Y + 273-443 AAV2 273-443 + 444 substitution 444 F Y + 445-499 AAV2 445-499 + 500 substitution 500 F Y + 501-699 AAV2 501-699 + 700 substitution 700 F Y + 701-703 AAV2 701-703 + 704 substitution 704 F Y + 705-729 AAV2 705-729 + 730 substitution 730 F Y + 731-735 AAV2 731-735 + diff --git a/test-par.html b/test-par.html new file mode 100644 index 0000000..1bf70f0 --- /dev/null +++ b/test-par.html @@ -0,0 +1,470 @@ + + +

+

Parental composition of sequence SCH2

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+SCH2         MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQ
+AAV6         ........................................................................................................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+SCH2         AKKRVLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVI
+AAV6         ........
+AAV9              ...................................................................................................................
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+SCH2         TTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSDYQLPYVLGSAH
+AAV8                                          ...........................................................................
+AAV9         .........................................................................                             ..................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+SCH2         EGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWL
+AAV2                                                             ....................................................................
+AAV9         .......................................................                                                                 
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+SCH2         PGPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQ
+AAV2         ........................................................................................................................
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+SCH2         GVLPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEG
+AAV2         ............................
+AAV9           ......................................................................................................................
+
+                    730
+SCH2         VYSEPRPIGTRYLTRNL
+AAV9         .................
+
+
+

Parental composition of sequence SCH9

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+SCH9         MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQ
+AAV6         ........................................................................................................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+SCH9         AKKRVLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVI
+AAV6         ........
+AAV9              ...................................................................................................................
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+SCH9         TTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSDYQLPYVLGSAH
+AAV8                                          ...........................................................................
+AAV9         .........................................................................                             ..................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+SCH9         EGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNWLP
+AAV2                                                                                                                            .....
+AAV9         .....................................................................................................................   
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+SCH9         GPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQG
+AAV2         ........................................................................................................................
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+SCH9         VLPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGV
+AAV2         ...........................
+AAV9          .......................................................................................................................
+
+                    730
+SCH9         YSEPRPIGTRYLTRNL
+AAV9         ................
+
+
+

Parental composition of sequence AAV-F

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+AAV-F        MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ
+AAV9         ........................................................................................................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+AAV-F        AKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVI
+AAV9         ........................................................................................................................
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+AAV-F        TTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDYQLPYVLGSAH
+AAV9         ........................................................................................................................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+AAV-F        EGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIP
+AAV9         ........................................................................................................................
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+AAV-F        GPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQFVVGQSYAQAQT
+AAV9         ............................................................................................................-------.....
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+AAV-F        GWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEF
+AAV9         ........................................................................................................................
+
+                    730       740
+AAV-F        AVNTEGVYSEPRPIGTRYLTRNL
+AAV9         .......................
+
+
+

Parental composition of sequence AAV-DJ

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+AAV-DJ       MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ
+AAV2         ........................................................................................................................
+AAV9                                                                                              ...................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+AAV-DJ       AKKRLLEPLGLVEEAAKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPIGEPPAAPSGVGSLTMAAGGGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI
+AAV2         ....            ...................................................                    .................................
+AAV8                                                                                          .....................                  
+AAV9         ...........................                                 ........................                               .....
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+AAV-DJ       TTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAH
+AAV2         .....................                                                                                                   
+AAV8                                          .......................................................................................
+AAV9         .........................................................................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+AAV-DJ       QGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFQFTYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQTTGGTTNTQTLGFSQGGPNTMANQAKNWL
+AAV2                                                                                                                              ...
+AAV8         ................................................................................................A.......................
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+AAV-DJ       PGPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQ
+AAV2         ........................................................................................................................
+AAV8         ...........                                                                                                             
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+AAV-DJ       GVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTTFNQSKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTSVDFAVNTEG
+AAV2         .........................................................
+AAV8                                      ...........................................................................................
+
+                    730
+AAV-DJ       VYSEPRPIGTRYLTRNL
+AAV8         .................
+
+
+

Parental composition of sequence AAV-PHP.B

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+AAV-PHP.B    MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ
+AAV9         ........................................................................................................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+AAV-PHP.B    AKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVI
+AAV9         ........................................................................................................................
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+AAV-PHP.B    TTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDYQLPYVLGSAH
+AAV9         ........................................................................................................................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+AAV-PHP.B    EGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIP
+AAV9         ........................................................................................................................
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+AAV-PHP.B    GPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQTLAVPFKAQAQT
+AAV9         ............................................................................................................-------.....
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+AAV-PHP.B    GWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEF
+AAV9         ........................................................................................................................
+
+                    730       740
+AAV-PHP.B    AVNTEGVYSEPRPIGTRYLTRNL
+AAV9         .......................
+
+
+

Parental composition of sequence AAV2.5T

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+AAV2.5T      MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ
+AAV2         ........................................................................................................................
+AAV5                                                                                                                             ....
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+AAV2.5T      AKKRVLEPFGLVEEGAKTAPTGKRIDDHFPKRKKARTEEDSKPSTSSDAEAGPSGSQQLQIPAQPASSLGADTMSAGGGGPLGDNNQGADGVGNASGDWHCDSTWMGDRVVTKSTRTWVL
+AAV2         ........
+AAV5         ........................................................................................................................
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+AAV2.5T      PSYNNHQYREIKSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLINNYWGFRPRSLRVKIFNIQVKEVTVQDSTTTIANNLTSTVQVFTDDDYQLPYVVGNGTEGCLPAFPPQ
+AAV5         ........................................................................................................................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+AAV2.5T      VFTLPQYGYATLNRDNTENPTERSSFFCLEYFPSKMLRTGNNFEFTYNFEEVPFHSSFAPSQNLFKLANPLVDQYLYRFVSTNNTGGVQFNKNLAGRYANTYKNWFPGPMGRTQGWNLGS
+AAV5         ........................................................................................................................
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+AAV2.5T      GVNRASVSAFATTNRMELEGASYQVPPQPNGMTNNLQGSNTYALENTMIFNSQPANPGTTATYLEGNMLITSESETQPVNRVAYNVGGQMATNNQSSTTAPTTGTYNLQEIVPGSVWMER
+AAV5         .....................................................................................................A..................
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+AAV2.5T      DVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPGNITSFSDVPVSSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNDPQFVDFAPDSTGEYRTTRPIGTRY
+AAV5         ........................................................................................................................
+
+AAV2.5T      LTRPL
+AAV5         .....
+
+
+

Parental composition of sequence 7m8

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+7m8          MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ
+AAV2         ........................................................................................................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+7m8          AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVT
+AAV2         .......................................................................................................................I
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+7m8          TTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG
+AAV2         ........................................................................................................................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+7m8          CLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPG
+AAV2         ........................................................................................................................
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+7m8          PCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNLALGETTRPARQA
+AAV2         ...........................................................................................................----------...
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+7m8          ATADVNTQGVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSINV
+AAV2         .....................................................................................................................V..
+
+                    730       740
+7m8          DFTVDTNGVYSEPRPIGTRYLTRNL
+AAV2         .........................
+
+
+

Parental composition of sequence LI-A

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+LI-A         MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ
+AAV2         ........................................................................................................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+LI-A         AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI
+AAV2         ........................................................................................................................
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+LI-A         TTSTRTWALPTYNNHLYKQISSASGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG
+AAV2         ......................Q.................................................................................................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+LI-A         CLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYFLSRTNSEGSLTTQSSLGFSQAGASDIRDQSRNWLPG
+AAV2         ...................................................................................Y.....TPSGT....R.Q...................
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+LI-A         PCYRQQRVSKTDGENNNSDFSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQGV
+AAV2         ...........SAD....EY....................................................................................................
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+LI-A         LPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSVNVDFTVDTNGVY
+AAV2         ........................................................................................................................
+
+                    730
+LI-A         SEPRPIGTRYLTRNL
+AAV2         ...............
+
+
+

Parental composition of sequence LI-C

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+LI-C         MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ
+AAV2         ........................................................................................................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+LI-C         AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI
+AAV2         ........................................................................................................................
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+LI-C         TTSTRTWALPTYNNHLYKQISSASGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG
+AAV2         ......................Q.................................................................................................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+LI-C         CLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPG
+AAV2         ........................................................................................................................
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+LI-C         PCYRQQRVSKTSADNNNSEFSWPGATTYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQGV
+AAV2         ...................Y..T...K.............................................................................................
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+LI-C         LPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSVNVDFTVDTNGVY
+AAV2         ........................................................................................................................
+
+                    730
+LI-C         SEPRPIGTRYLTRNL
+AAV2         ...............
+
+
+

Parental composition of sequence NP84

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+NP84         MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ
+AAV2         ........................................................................................................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+NP84         AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKTGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSQWLGDRVI
+AAV2         .........................................                             ..........................................        
+AAV3B                                                                                               .................................
+AAV6                                                ..................................
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+NP84         TTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG
+AAV2                                                                                 ................................................
+AAV3B        ........................................................................................................................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+NP84         CLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPG
+AAV2         ........................................................................................................................
+AAV3B        .................................................
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+NP84         PCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEEFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQGGNRQAATADVNTQGV
+AAV2         ...................................................K....................................................R...............
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+NP84         LPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSVNVDFTVDTNGVY
+AAV2         ........................................................................................................................
+
+                    730
+NP84         SEPRPIGTRYLTRNL
+AAV2         ...............
+
+
+

Parental composition of sequence OLIG001

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+OLIG001      MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ
+AAV2         ........................................................................................................................
+AAV9                                                                                              ...................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+OLIG001      AKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVI
+AAV2         ....                                                                                                                    
+AAV6                        ...........................................                                                              
+AAV8                                                                                                                          .......
+AAV9         ....................................        ............................................................................
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+OLIG001      TTSTRTWALPTYNNHLYKQISNGTSGGATNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAH
+AAV2                                                                                                     ............................
+AAV8         ..................................................................................................... ..................
+AAV9         ......................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+OLIG001      QGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFQFTYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQTTGGTANTQTLGFSQGGPNTMANQAKNWL
+AAV2         .............
+AAV8         ........................................................................................................................
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+OLIG001      PGPCYRQQRVSTTTGQNNNSNFAWTAGTKYHLNGRNSLANPGIAMATHKDDKERFFPSNGILIFGKQNAARDNADYSDVMLTSEEEIKTTNPVATEEYGIVADNLQQQNTAPQIGTVNSQ
+AAV8         ...................................................E....................................................................
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+OLIG001      GALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTTFNQSKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTSVDFAVNTEG
+AAV8         ........................................................................................................................
+
+                    730
+OLIG001      VYSEPHPIGTRYLTRPL
+AAV6               ...........
+AAV8         .....
+
+
+

Parental composition of sequence AAV2i8

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+AAV2i8       MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ
+AAV2         ........................................................................................................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+AAV2i8       AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI
+AAV2         ........................................................................................................................
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+AAV2i8       TTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG
+AAV2         ........................................................................................................................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+AAV2i8       CLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPG
+AAV2         ........................................................................................................................
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+AAV2i8       PCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQQQNTAPATADVNTQGV
+AAV2         ........................................................................................................      ..........
+AAV8                                                                                                              .........
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+AAV2i8       LPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSVNVDFTVDTNGVY
+AAV2         ........................................................................................................................
+
+                    730
+AAV2i8       SEPRPIGTRYLTRNL
+AAV2         ...............
+
+
+

Parental composition of sequence rAAV2-retro

+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+rAAV2-retro  MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ
+AAV2         ........................................................................................................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+rAAV2-retro  AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI
+AAV2         ........................................................................................................................
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+rAAV2-retro  TTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG
+AAV2         ........................................................................................................................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+rAAV2-retro  CLPPFPADVFMVPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPG
+AAV2         .....................N..................................................................................................
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+rAAV2-retro  PCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNLADQDYTKTARQA
+AAV2         ...........................................................................................................----------...
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+rAAV2-retro  ATADVNTQGVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSINV
+AAV2         .....................................................................................................................V..
+
+                    730       740
+rAAV2-retro  DFTVDTNGVYSEPRPIGTRYLTRNL
+AAV2         .........................
+
+
+

Parental composition of sequence AAV2-sept-Y-F

+Sequence name too long, replaced with S14 (the number indicates sequence position in the variant file)
+
+                     10        20        30        40        50        60        70        80        90       100       110       120
+S14          MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ
+AAV2         ........................................................................................................................
+
+                    130       140       150       160       170       180       190       200       210       220       230       240
+S14          AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI
+AAV2         ........................................................................................................................
+
+                    250       260       270       280       290       300       310       320       330       340       350       360
+S14          TTSTRTWALPTFNNHLYKQISSQSGASNDNHFFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG
+AAV2         ...........Y...................Y........................................................................................
+
+                    370       380       390       400       410       420       430       440       450       460       470       480
+S14          CLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYFLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPG
+AAV2         ...................................................................................Y....................................
+
+                    490       500       510       520       530       540       550       560       570       580       590       600
+S14          PCYRQQRVSKTSADNNNSEFSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQGV
+AAV2         ...................Y....................................................................................................
+
+                    610       620       630       640       650       660       670       680       690       700       710       720
+S14          LPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQFTSNFNKSVNVDFTVDTNGVY
+AAV2         ...................................................................................................Y...Y................
+
+                    730
+S14          SEPRPIGTRFLTRNL
+AAV2         .........Y.....
+
+
+

+ diff --git a/test-par.txt b/test-par.txt new file mode 100644 index 0000000..d9f3b82 --- /dev/null +++ b/test-par.txt @@ -0,0 +1,492 @@ +Parental composition of sequence SCH2 + + 10 20 30 40 50 60 70 80 90 100 110 120 +SCH2 MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQ +AAV2 +AAV6 ........................................................................................................................ +AAV8 +AAV9 + + 130 140 150 160 170 180 190 200 210 220 230 240 +SCH2 AKKRVLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVI +AAV2 +AAV6 ........ +AAV8 +AAV9 ................................................................................................................... + + 250 260 270 280 290 300 310 320 330 340 350 360 +SCH2 TTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSDYQLPYVLGSAH +AAV2 +AAV8 ........................................................................... +AAV9 ......................................................................... .................. + + 370 380 390 400 410 420 430 440 450 460 470 480 +SCH2 EGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWL +AAV2 .................................................................... +AAV9 ....................................................... + + 490 500 510 520 530 540 550 560 570 580 590 600 +SCH2 PGPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQ +AAV2 ........................................................................................................................ +AAV9 + + 610 620 630 640 650 660 670 680 690 700 710 720 +SCH2 GVLPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEG +AAV2 ............................ +AAV9 ...................................................................................................................... + + 730 +SCH2 VYSEPRPIGTRYLTRNL +AAV9 ................. + + +Parental composition of sequence SCH9 + + 10 20 30 40 50 60 70 80 90 100 110 120 +SCH9 MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQ +AAV2 +AAV6 ........................................................................................................................ +AAV8 +AAV9 + + 130 140 150 160 170 180 190 200 210 220 230 240 +SCH9 AKKRVLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVI +AAV2 +AAV6 ........ +AAV8 +AAV9 ................................................................................................................... + + 250 260 270 280 290 300 310 320 330 340 350 360 +SCH9 TTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSDYQLPYVLGSAH +AAV2 +AAV8 ........................................................................... +AAV9 ......................................................................... .................. + + 370 380 390 400 410 420 430 440 450 460 470 480 +SCH9 EGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNWLP +AAV2 ..... +AAV9 ..................................................................................................................... + + 490 500 510 520 530 540 550 560 570 580 590 600 +SCH9 GPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQG +AAV2 ........................................................................................................................ +AAV9 + + 610 620 630 640 650 660 670 680 690 700 710 720 +SCH9 VLPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGV +AAV2 ........................... +AAV9 ....................................................................................................................... + + 730 +SCH9 YSEPRPIGTRYLTRNL +AAV9 ................ + + +Parental composition of sequence AAV-F + + 10 20 30 40 50 60 70 80 90 100 110 120 +AAV-F MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ +AAV9 ........................................................................................................................ + + 130 140 150 160 170 180 190 200 210 220 230 240 +AAV-F AKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVI +AAV9 ........................................................................................................................ + + 250 260 270 280 290 300 310 320 330 340 350 360 +AAV-F TTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDYQLPYVLGSAH +AAV9 ........................................................................................................................ + + 370 380 390 400 410 420 430 440 450 460 470 480 +AAV-F EGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIP +AAV9 ........................................................................................................................ + + 490 500 510 520 530 540 550 560 570 580 590 600 +AAV-F GPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQFVVGQSYAQAQT +AAV9 ............................................................................................................-------..... + + 610 620 630 640 650 660 670 680 690 700 710 720 +AAV-F GWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEF +AAV9 ........................................................................................................................ + + 730 740 +AAV-F AVNTEGVYSEPRPIGTRYLTRNL +AAV9 ....................... + + +Parental composition of sequence AAV-DJ + + 10 20 30 40 50 60 70 80 90 100 110 120 +AAV-DJ MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ +AAV2 ........................................................................................................................ +AAV8 +AAV9 ................................... + + 130 140 150 160 170 180 190 200 210 220 230 240 +AAV-DJ AKKRLLEPLGLVEEAAKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPIGEPPAAPSGVGSLTMAAGGGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI +AAV2 .... ................................................... ................................. +AAV8 ..................... +AAV9 ........................... ........................ ..... + + 250 260 270 280 290 300 310 320 330 340 350 360 +AAV-DJ TTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAH +AAV2 ..................... +AAV8 ....................................................................................... +AAV9 ......................................................................... + + 370 380 390 400 410 420 430 440 450 460 470 480 +AAV-DJ QGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFQFTYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQTTGGTTNTQTLGFSQGGPNTMANQAKNWL +AAV2 ... +AAV8 ................................................................................................A....................... + + 490 500 510 520 530 540 550 560 570 580 590 600 +AAV-DJ PGPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQ +AAV2 ........................................................................................................................ +AAV8 ........... + + 610 620 630 640 650 660 670 680 690 700 710 720 +AAV-DJ GVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTTFNQSKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTSVDFAVNTEG +AAV2 ......................................................... +AAV8 ........................................................................................... + + 730 +AAV-DJ VYSEPRPIGTRYLTRNL +AAV8 ................. + + +Parental composition of sequence AAV-PHP.B + + 10 20 30 40 50 60 70 80 90 100 110 120 +AAV-PHP.B MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ +AAV9 ........................................................................................................................ + + 130 140 150 160 170 180 190 200 210 220 230 240 +AAV-PHP.B AKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVI +AAV9 ........................................................................................................................ + + 250 260 270 280 290 300 310 320 330 340 350 360 +AAV-PHP.B TTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDYQLPYVLGSAH +AAV9 ........................................................................................................................ + + 370 380 390 400 410 420 430 440 450 460 470 480 +AAV-PHP.B EGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIP +AAV9 ........................................................................................................................ + + 490 500 510 520 530 540 550 560 570 580 590 600 +AAV-PHP.B GPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQTLAVPFKAQAQT +AAV9 ............................................................................................................-------..... + + 610 620 630 640 650 660 670 680 690 700 710 720 +AAV-PHP.B GWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEF +AAV9 ........................................................................................................................ + + 730 740 +AAV-PHP.B AVNTEGVYSEPRPIGTRYLTRNL +AAV9 ....................... + + +Parental composition of sequence AAV2.5T + + 10 20 30 40 50 60 70 80 90 100 110 120 +AAV2.5T MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ +AAV2 ........................................................................................................................ +AAV5 .... + + 130 140 150 160 170 180 190 200 210 220 230 240 +AAV2.5T AKKRVLEPFGLVEEGAKTAPTGKRIDDHFPKRKKARTEEDSKPSTSSDAEAGPSGSQQLQIPAQPASSLGADTMSAGGGGPLGDNNQGADGVGNASGDWHCDSTWMGDRVVTKSTRTWVL +AAV2 ........ +AAV5 ........................................................................................................................ + + 250 260 270 280 290 300 310 320 330 340 350 360 +AAV2.5T PSYNNHQYREIKSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLINNYWGFRPRSLRVKIFNIQVKEVTVQDSTTTIANNLTSTVQVFTDDDYQLPYVVGNGTEGCLPAFPPQ +AAV5 ........................................................................................................................ + + 370 380 390 400 410 420 430 440 450 460 470 480 +AAV2.5T VFTLPQYGYATLNRDNTENPTERSSFFCLEYFPSKMLRTGNNFEFTYNFEEVPFHSSFAPSQNLFKLANPLVDQYLYRFVSTNNTGGVQFNKNLAGRYANTYKNWFPGPMGRTQGWNLGS +AAV5 ........................................................................................................................ + + 490 500 510 520 530 540 550 560 570 580 590 600 +AAV2.5T GVNRASVSAFATTNRMELEGASYQVPPQPNGMTNNLQGSNTYALENTMIFNSQPANPGTTATYLEGNMLITSESETQPVNRVAYNVGGQMATNNQSSTTAPTTGTYNLQEIVPGSVWMER +AAV5 .....................................................................................................A.................. + + 610 620 630 640 650 660 670 680 690 700 710 720 +AAV2.5T DVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPGNITSFSDVPVSSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNDPQFVDFAPDSTGEYRTTRPIGTRY +AAV5 ........................................................................................................................ + +AAV2.5T LTRPL +AAV5 ..... + + +Parental composition of sequence 7m8 + + 10 20 30 40 50 60 70 80 90 100 110 120 +7m8 MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ +AAV2 ........................................................................................................................ + + 130 140 150 160 170 180 190 200 210 220 230 240 +7m8 AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVT +AAV2 .......................................................................................................................I + + 250 260 270 280 290 300 310 320 330 340 350 360 +7m8 TTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG +AAV2 ........................................................................................................................ + + 370 380 390 400 410 420 430 440 450 460 470 480 +7m8 CLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPG +AAV2 ........................................................................................................................ + + 490 500 510 520 530 540 550 560 570 580 590 600 +7m8 PCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNLALGETTRPARQA +AAV2 ...........................................................................................................----------... + + 610 620 630 640 650 660 670 680 690 700 710 720 +7m8 ATADVNTQGVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSINV +AAV2 .....................................................................................................................V.. + + 730 740 +7m8 DFTVDTNGVYSEPRPIGTRYLTRNL +AAV2 ......................... + + +Parental composition of sequence LI-A + + 10 20 30 40 50 60 70 80 90 100 110 120 +LI-A MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ +AAV2 ........................................................................................................................ + + 130 140 150 160 170 180 190 200 210 220 230 240 +LI-A AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI +AAV2 ........................................................................................................................ + + 250 260 270 280 290 300 310 320 330 340 350 360 +LI-A TTSTRTWALPTYNNHLYKQISSASGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG +AAV2 ......................Q................................................................................................. + + 370 380 390 400 410 420 430 440 450 460 470 480 +LI-A CLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYFLSRTNSEGSLTTQSSLGFSQAGASDIRDQSRNWLPG +AAV2 ...................................................................................Y.....TPSGT....R.Q................... + + 490 500 510 520 530 540 550 560 570 580 590 600 +LI-A PCYRQQRVSKTDGENNNSDFSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQGV +AAV2 ...........SAD....EY.................................................................................................... + + 610 620 630 640 650 660 670 680 690 700 710 720 +LI-A LPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSVNVDFTVDTNGVY +AAV2 ........................................................................................................................ + + 730 +LI-A SEPRPIGTRYLTRNL +AAV2 ............... + + +Parental composition of sequence LI-C + + 10 20 30 40 50 60 70 80 90 100 110 120 +LI-C MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ +AAV2 ........................................................................................................................ + + 130 140 150 160 170 180 190 200 210 220 230 240 +LI-C AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI +AAV2 ........................................................................................................................ + + 250 260 270 280 290 300 310 320 330 340 350 360 +LI-C TTSTRTWALPTYNNHLYKQISSASGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG +AAV2 ......................Q................................................................................................. + + 370 380 390 400 410 420 430 440 450 460 470 480 +LI-C CLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPG +AAV2 ........................................................................................................................ + + 490 500 510 520 530 540 550 560 570 580 590 600 +LI-C PCYRQQRVSKTSADNNNSEFSWPGATTYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQGV +AAV2 ...................Y..T...K............................................................................................. + + 610 620 630 640 650 660 670 680 690 700 710 720 +LI-C LPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSVNVDFTVDTNGVY +AAV2 ........................................................................................................................ + + 730 +LI-C SEPRPIGTRYLTRNL +AAV2 ............... + + +Parental composition of sequence NP84 + + 10 20 30 40 50 60 70 80 90 100 110 120 +NP84 MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ +AAV2 ........................................................................................................................ +AAV3B +AAV6 + + 130 140 150 160 170 180 190 200 210 220 230 240 +NP84 AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKTGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSQWLGDRVI +AAV2 ......................................... .......................................... +AAV3B ................................. +AAV6 .................................. + + 250 260 270 280 290 300 310 320 330 340 350 360 +NP84 TTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG +AAV2 ................................................ +AAV3B ........................................................................................................................ + + 370 380 390 400 410 420 430 440 450 460 470 480 +NP84 CLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPG +AAV2 ........................................................................................................................ +AAV3B ................................................. + + 490 500 510 520 530 540 550 560 570 580 590 600 +NP84 PCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEEFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQGGNRQAATADVNTQGV +AAV2 ...................................................K....................................................R............... + + 610 620 630 640 650 660 670 680 690 700 710 720 +NP84 LPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSVNVDFTVDTNGVY +AAV2 ........................................................................................................................ + + 730 +NP84 SEPRPIGTRYLTRNL +AAV2 ............... + + +Parental composition of sequence OLIG001 + + 10 20 30 40 50 60 70 80 90 100 110 120 +OLIG001 MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ +AAV2 ........................................................................................................................ +AAV6 +AAV8 +AAV9 ................................... + + 130 140 150 160 170 180 190 200 210 220 230 240 +OLIG001 AKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVI +AAV2 .... +AAV6 ........................................... +AAV8 ....... +AAV9 .................................... ............................................................................ + + 250 260 270 280 290 300 310 320 330 340 350 360 +OLIG001 TTSTRTWALPTYNNHLYKQISNGTSGGATNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAH +AAV2 ............................ +AAV6 +AAV8 ..................................................................................................... .................. +AAV9 ...................... + + 370 380 390 400 410 420 430 440 450 460 470 480 +OLIG001 QGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFQFTYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQTTGGTANTQTLGFSQGGPNTMANQAKNWL +AAV2 ............. +AAV6 +AAV8 ........................................................................................................................ + + 490 500 510 520 530 540 550 560 570 580 590 600 +OLIG001 PGPCYRQQRVSTTTGQNNNSNFAWTAGTKYHLNGRNSLANPGIAMATHKDDKERFFPSNGILIFGKQNAARDNADYSDVMLTSEEEIKTTNPVATEEYGIVADNLQQQNTAPQIGTVNSQ +AAV6 +AAV8 ...................................................E.................................................................... + + 610 620 630 640 650 660 670 680 690 700 710 720 +OLIG001 GALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTTFNQSKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTSVDFAVNTEG +AAV6 +AAV8 ........................................................................................................................ + + 730 +OLIG001 VYSEPHPIGTRYLTRPL +AAV6 ........... +AAV8 ..... + + +Parental composition of sequence AAV2i8 + + 10 20 30 40 50 60 70 80 90 100 110 120 +AAV2i8 MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ +AAV2 ........................................................................................................................ +AAV8 + + 130 140 150 160 170 180 190 200 210 220 230 240 +AAV2i8 AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI +AAV2 ........................................................................................................................ +AAV8 + + 250 260 270 280 290 300 310 320 330 340 350 360 +AAV2i8 TTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG +AAV2 ........................................................................................................................ +AAV8 + + 370 380 390 400 410 420 430 440 450 460 470 480 +AAV2i8 CLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPG +AAV2 ........................................................................................................................ +AAV8 + + 490 500 510 520 530 540 550 560 570 580 590 600 +AAV2i8 PCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQQQNTAPATADVNTQGV +AAV2 ........................................................................................................ .......... +AAV8 ......... + + 610 620 630 640 650 660 670 680 690 700 710 720 +AAV2i8 LPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSVNVDFTVDTNGVY +AAV2 ........................................................................................................................ + + 730 +AAV2i8 SEPRPIGTRYLTRNL +AAV2 ............... + + +Parental composition of sequence rAAV2-retro + + 10 20 30 40 50 60 70 80 90 100 110 120 +rAAV2-retro MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ +AAV2 ........................................................................................................................ + + 130 140 150 160 170 180 190 200 210 220 230 240 +rAAV2-retro AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI +AAV2 ........................................................................................................................ + + 250 260 270 280 290 300 310 320 330 340 350 360 +rAAV2-retro TTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG +AAV2 ........................................................................................................................ + + 370 380 390 400 410 420 430 440 450 460 470 480 +rAAV2-retro CLPPFPADVFMVPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPG +AAV2 .....................N.................................................................................................. + + 490 500 510 520 530 540 550 560 570 580 590 600 +rAAV2-retro PCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNLADQDYTKTARQA +AAV2 ...........................................................................................................----------... + + 610 620 630 640 650 660 670 680 690 700 710 720 +rAAV2-retro ATADVNTQGVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSINV +AAV2 .....................................................................................................................V.. + + 730 740 +rAAV2-retro DFTVDTNGVYSEPRPIGTRYLTRNL +AAV2 ......................... + + +Parental composition of sequence AAV2-sept-Y-F +Sequence name too long, replaced with S14 (the number indicates sequence position in the variant file) + + 10 20 30 40 50 60 70 80 90 100 110 120 +S14 MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ +AAV2 ........................................................................................................................ + + 130 140 150 160 170 180 190 200 210 220 230 240 +S14 AKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI +AAV2 ........................................................................................................................ + + 250 260 270 280 290 300 310 320 330 340 350 360 +S14 TTSTRTWALPTFNNHLYKQISSQSGASNDNHFFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQG +AAV2 ...........Y...................Y........................................................................................ + + 370 380 390 400 410 420 430 440 450 460 470 480 +S14 CLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYFLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPG +AAV2 ...................................................................................Y.................................... + + 490 500 510 520 530 540 550 560 570 580 590 600 +S14 PCYRQQRVSKTSADNNNSEFSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQGV +AAV2 ...................Y.................................................................................................... + + 610 620 630 640 650 660 670 680 690 700 710 720 +S14 LPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQFTSNFNKSVNVDFTVDTNGVY +AAV2 ...................................................................................................Y...Y................ + + 730 +S14 SEPRPIGTRFLTRNL +AAV2 .........Y..... + + diff --git a/test-stats.txt b/test-stats.txt new file mode 100644 index 0000000..9347474 --- /dev/null +++ b/test-stats.txt @@ -0,0 +1,17 @@ + Length Parents Main Coverage Matches ID% Identities Ins_sites Ins Del_sites Dels Subs Other +Name +LI-A 735 1 AAV2 98.10 721 98.10 721 0 0 0 0 14 0 +LI-C 735 1 AAV2 99.46 731 99.46 731 0 0 0 0 4 0 +S14 735 1 AAV2 99.05 728 99.05 728 0 0 0 0 7 0 +7m8 745 1 AAV2 98.39 733 98.39 733 1 10 0 0 2 0 +rAAV2-retro 745 1 AAV2 98.39 733 98.39 733 1 10 0 0 2 0 +AAV-F 743 1 AAV9 99.06 736 99.06 736 1 7 0 0 0 0 +AAV-PHP.B 743 1 AAV9 99.06 736 99.06 736 1 7 0 0 0 0 +AAV2i8 735 2 AAV2 99.18 729 99.32 730 0 0 0 0 0 0 +AAV2.5T 725 2 AAV5 83.86 608 94.21 683 0 0 0 0 1 0 +AAV-DJ 737 3 AAV2 55.50 409 92.94 685 0 0 0 0 1 0 +NP84 735 3 AAV2 84.90 624 98.50 724 0 0 0 0 2 0 +OLIG001 737 4 AAV8 66.49 490 95.12 701 0 0 0 0 1 1 +SCH2 737 4 AAV9 53.73 396 89.55 660 0 0 0 0 0 0 +SCH9 736 4 AAV9 62.23 458 92.12 678 0 0 0 0 0 0 +

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