Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Allow reverse complement matching? #19

Open
mschubert opened this issue Feb 20, 2023 · 0 comments
Open

Allow reverse complement matching? #19

mschubert opened this issue Feb 20, 2023 · 0 comments

Comments

@mschubert
Copy link

My barcode library is read using only a specific sequencing primer that anneals to the reverse strand. As such, all reads that my FASTQs contain will be the reverse complement of the known barcode, not the barcode itself.

I can of course manually reverse complement my library to achieve the same effect, but would it make sense to provide a --reverse-complement option for such use cases?

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant