You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
My barcode library is read using only a specific sequencing primer that anneals to the reverse strand. As such, all reads that my FASTQs contain will be the reverse complement of the known barcode, not the barcode itself.
I can of course manually reverse complement my library to achieve the same effect, but would it make sense to provide a --reverse-complement option for such use cases?
The text was updated successfully, but these errors were encountered:
My barcode library is read using only a specific sequencing primer that anneals to the reverse strand. As such, all reads that my FASTQs contain will be the reverse complement of the known barcode, not the barcode itself.
I can of course manually reverse complement my library to achieve the same effect, but would it make sense to provide a
--reverse-complement
option for such use cases?The text was updated successfully, but these errors were encountered: