From 955701d62f61ddb2a2fac23ab8e0dc3da797985a Mon Sep 17 00:00:00 2001 From: Lucille Delisle Date: Tue, 3 Oct 2023 15:02:20 +0200 Subject: [PATCH 01/12] add baredSC_1d --- .../transcriptomics/baredsc/.dockstore.yml | 9 + .../transcriptomics/baredsc/CHANGELOG.md | 5 + workflows/transcriptomics/baredsc/README.md | 19 + .../baredsc/baredSC_1d_logNorm.ga | 603 +++++ .../baredsc/baredSC_1d_logNorm_tests.yml | 160 ++ .../baredsc/test-data/combined_1d_plot.png | Bin 0 -> 226847 bytes .../test-data/nih3t3_generated_2d_2.txt | 2362 +++++++++++++++++ 7 files changed, 3158 insertions(+) create mode 100644 workflows/transcriptomics/baredsc/.dockstore.yml create mode 100644 workflows/transcriptomics/baredsc/CHANGELOG.md create mode 100644 workflows/transcriptomics/baredsc/README.md create mode 100644 workflows/transcriptomics/baredsc/baredSC_1d_logNorm.ga create mode 100644 workflows/transcriptomics/baredsc/baredSC_1d_logNorm_tests.yml create mode 100644 workflows/transcriptomics/baredsc/test-data/combined_1d_plot.png create mode 100644 workflows/transcriptomics/baredsc/test-data/nih3t3_generated_2d_2.txt diff --git a/workflows/transcriptomics/baredsc/.dockstore.yml b/workflows/transcriptomics/baredsc/.dockstore.yml new file mode 100644 index 000000000..54602e951 --- /dev/null +++ b/workflows/transcriptomics/baredsc/.dockstore.yml @@ -0,0 +1,9 @@ +version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /baredSC_1d_logNorm.ga + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 diff --git a/workflows/transcriptomics/baredsc/CHANGELOG.md b/workflows/transcriptomics/baredsc/CHANGELOG.md new file mode 100644 index 000000000..782e1a24d --- /dev/null +++ b/workflows/transcriptomics/baredsc/CHANGELOG.md @@ -0,0 +1,5 @@ +# Changelog + +## [0.1] 2023-10-03 + +First release. diff --git a/workflows/transcriptomics/baredsc/README.md b/workflows/transcriptomics/baredsc/README.md new file mode 100644 index 000000000..2e98be41a --- /dev/null +++ b/workflows/transcriptomics/baredsc/README.md @@ -0,0 +1,19 @@ +# BaredSC Workflows + +These workflows allow to run a baredSC analysis in a single click. It uses models from 1 to N Gaussians and combine them. + +## Inputs dataset + +- The workflow needs a tabular dataset where each row is a cell. The tabular needs to have a header line with column names. There must be at least two columns: 'nCount_RNA' and another one with the counts for the gene of interest. + +## Inputs values + +- Gene name: The name of the column with the counts of your gene of interest. +- Maximum value in logNorm: The maximum value to explore in PDF. This value should be large enough so the PDF is at 0 at this value. +- Maximum number of Gaussians to study: All models between models with 1 Gaussians to models with this number of Gaussians. + +## Processing + +- The workflow will generate paramater values from 1 to the maximum number of Gaussians to study. +- baredSC_1d is run for each of these number of Gaussians +- All models are combined into a single result. diff --git a/workflows/transcriptomics/baredsc/baredSC_1d_logNorm.ga b/workflows/transcriptomics/baredsc/baredSC_1d_logNorm.ga new file mode 100644 index 000000000..915dcf17d --- /dev/null +++ b/workflows/transcriptomics/baredsc/baredSC_1d_logNorm.ga @@ -0,0 +1,603 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "Run baredSC in logNorm for 1 to N gaussians and combine models.", + "creator": [ + { + "class": "Person", + "identifier": "https://orcid.org/0000-0002-1964-4960", + "name": "Lucille Delisle" + } + ], + "format-version": "0.1", + "license": "MIT", + "name": "baredSC_1d_logNorm", + "steps": { + "0": { + "annotation": "The dataset must have a first row with row names. 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outputs: + baredsc_numpy: + element_tests: + split_file_000000.tabular: + asserts: + size: 1257919 + delta_frac: 0.1 + split_file_000001.tabular: + asserts: + size: 1601519 + delta_frac: 0.1 + split_file_000002.tabular: + asserts: + size: 2180423 + delta_frac: 0.1 + split_file_000003.tabular: + asserts: + size: 28234812 + delta_frac: 0.1 + split_file_000003.tabular: + baredsc_qc_plots: + element_tests: + split_file_000000.tabular: + element_tests: + convergence: + asserts: + size: 20304 + delta_frac: 0.1 + corner: + asserts: + size: 63603 + delta_frac: 0.1 + p: + asserts: + size: 45302 + delta_frac: 0.1 + split_file_000001.tabular: + element_tests: + convergence: + asserts: + size: 27704 + delta_frac: 0.1 + corner: + asserts: + size: 387365 + delta_frac: 0.1 + p: + asserts: + size: 97623 + delta_frac: 0.1 + split_file_000002.tabular: + element_tests: + convergence: + asserts: + size: 32463 + delta_frac: 0.1 + corner: + asserts: + size: 1103840 + delta_frac: 0.1 + p: + asserts: + size: 159797 + delta_frac: 0.1 + split_file_000003.tabular: + element_tests: + convergence: + asserts: + size: 31343 + delta_frac: 0.1 + corner: + asserts: + size: 2139411 + delta_frac: 0.1 + p: + asserts: + size: 175774 + delta_frac: 0.1 + baredsc_neff: + element_tests: + split_file_000000.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "80[0-9][0-9]\.[0-9]*" + split_file_000001.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "13[0-9][0-9]\.[0-9]*" + split_file_000002.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "29[0-9]\.[0-9]*" + split_file_000003.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "78[0-9]\.[0-9]*" + combined_other_outputs: + element_tests: + individuals: + asserts: + has_size: + size: 108407 + delta_frac: 0.1 + means: + asserts: + has_n_lines: + n: 99998 + has_line_matching: + expression: "6\.[0-9]*e-01" + posterior_andco: + asserts: + has_size: + size: 197980 + delta_frac: 0.1 + posterior_individuals: + asserts: + has_size: + size: 105262 + delta_frac: 0.1 + posterior_per_cell: + asserts: + has_n_lines: + n: 2632 + has_line_matching: + exoression: "mu\tsd" + with_posterior: + asserts: + has_size: + size: 234303 + delta_frac: 0.1 + combined_pdf: + asserts: + that: + - has_line: + line: "x\tlow\tmean\thigh\tmedian" + - has_text: + text: "0.0125\t" + combined_plot: + path: test-data/combined_1d_plot.png + compare: sim_size + delta_frac: 0.1 + + \ No newline at end of file diff --git a/workflows/transcriptomics/baredsc/test-data/combined_1d_plot.png b/workflows/transcriptomics/baredsc/test-data/combined_1d_plot.png new file mode 100644 index 0000000000000000000000000000000000000000..507c0868fa79807d6bffe026dacd578ee1d4873b GIT binary patch literal 226847 zcmeFZdpMNq_dot1l+gB0D5AsOQgUc3jB`6u36Vn%4P}=QgK-+CnYLX-Q5z+vkz-6w zIUi>_DyLBzVlW~m<7|x6n3>=GjQ#n2Kfmkx{r|hJ-}QD~)#Q1e`?>G6*1gtiz1F(t 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0.46673405966078196 0.9710969217818207 1.0 +13561 group3 0 2 1.0787246582949683 0.9841428767980445 3.0 1 0 0.8570738517467593 0.3413881658359805 2.0 +11390 group3 2 2 0.9604294894508263 1.2253630529191466 3.0 0 0 1.012756884988206 0.44265515147011836 2.0 +55708 group1 8 12 0.8733377369334027 0.9801556173055537 3.0 1 9 0.3070577902385216 0.9513703345871919 1.0 +16063 group3 2 1 0.9644819197446499 0.8783361692912572 3.0 2 1 1.085734241123231 0.4196712229928285 2.0 +16422 group3 1 0 0.5406844787097789 0.3384544586482739 0.0 2 6 0.5391804026148392 0.9567956910514819 1.0 +17668 group3 4 6 1.0799805445421096 1.031096050631378 3.0 0 2 0.39168343939938344 0.9868614621641726 1.0 +16415 group3 1 4 0.9923551015783658 1.0198173980788054 3.0 1 7 0.28043205411160976 1.2111948741909506 1.0 +16653 group3 3 1 0.7949225542667253 1.0857032601068828 3.0 6 2 1.0650583476769155 0.3033123505712213 2.0 +6630 group1 3 1 1.052039376110604 1.0196469054881838 3.0 0 1 0.9599728103261282 0.27691535289423685 2.0 +7997 group3 2 3 0.9373885579676389 1.0929122783305962 3.0 0 3 0.4138438514343339 1.0972371520000848 1.0 +16842 group2 2 1 1.0123686877389662 1.0138181807538984 3.0 4 1 1.0386558479472863 0.29642211452153533 2.0 +15418 group3 0 0 0.4977397972254476 0.29342809208998816 0.0 0 3 0.43103048686992385 1.0884920273765328 1.0 +16977 group3 4 3 0.9812798602404847 1.1004306057391895 3.0 2 5 0.44708787541087025 1.052007688880996 1.0 +8249 group3 0 0 0.24398068905795048 0.4446066052836349 0.0 3 1 1.0972131920857282 0.5432789321063332 2.0 +38624 group2 4 5 0.914525673837027 1.0025201921010993 3.0 3 9 0.6126885981267824 0.9966513382000214 1.0 +13713 group3 0 0 0.3508954425341463 0.14396342558201491 0.0 1 0 0.5455349699532419 1.0157638758893444 1.0 +14578 group2 0 0 0.3855539972318272 0.27075459163213833 0.0 1 0 0.9670287407119521 0.3666579233697094 2.0 +12152 group3 0 0 0.2933301214987318 0.23591015760260986 0.0 3 2 0.8573550195271602 0.4908410106271917 2.0 +28796 group1 0 2 0.39605190921232225 0.31741453320872637 0.0 1 0 0.3478635238179973 0.8191960715673223 1.0 +15427 group3 2 1 0.9733283804040067 1.0062014237283545 3.0 0 1 0.3537778336845988 0.9286706453933524 1.0 +16065 group3 1 2 0.9379392206019438 1.0045767370103011 3.0 0 5 0.12951255367862535 0.8870814948670983 1.0 +15383 group2 1 4 1.009630850730726 1.0807864197771637 3.0 2 1 0.34921474837059063 1.0488463942447201 1.0 From e3ccae04e117875438d2ed17da957e1682065c05 Mon Sep 17 00:00:00 2001 From: Lucille Delisle Date: Tue, 3 Oct 2023 15:08:57 +0200 Subject: [PATCH 02/12] remove \. --- .../baredsc/baredSC_1d_logNorm_tests.yml | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/workflows/transcriptomics/baredsc/baredSC_1d_logNorm_tests.yml b/workflows/transcriptomics/baredsc/baredSC_1d_logNorm_tests.yml index 8dc7ffabe..9894e4440 100644 --- a/workflows/transcriptomics/baredsc/baredSC_1d_logNorm_tests.yml +++ b/workflows/transcriptomics/baredsc/baredSC_1d_logNorm_tests.yml @@ -92,25 +92,25 @@ has_n_lines: n: 1 has_line_matching: - expression: "80[0-9][0-9]\.[0-9]*" + expression: "80[0-9][0-9].[0-9]*" split_file_000001.tabular: asserts: has_n_lines: n: 1 has_line_matching: - expression: "13[0-9][0-9]\.[0-9]*" + expression: "13[0-9][0-9].[0-9]*" split_file_000002.tabular: asserts: has_n_lines: n: 1 has_line_matching: - expression: "29[0-9]\.[0-9]*" + expression: "29[0-9].[0-9]*" split_file_000003.tabular: asserts: has_n_lines: n: 1 has_line_matching: - expression: "78[0-9]\.[0-9]*" + expression: "78[0-9].[0-9]*" combined_other_outputs: element_tests: individuals: @@ -123,7 +123,7 @@ has_n_lines: n: 99998 has_line_matching: - expression: "6\.[0-9]*e-01" + expression: "6.[0-9]*e-01" posterior_andco: asserts: has_size: From 9ac08474b22909b5db75c60647bc079c84258680 Mon Sep 17 00:00:00 2001 From: Lucille Delisle Date: Tue, 3 Oct 2023 15:38:53 +0200 Subject: [PATCH 03/12] change directory + fix test syntax --- .../baredsc/.dockstore.yml | 0 .../baredsc/CHANGELOG.md | 0 .../baredsc/README.md | 0 .../baredsc/baredSC_1d_logNorm.ga | 6 +- .../baredsc/baredSC_1d_logNorm_tests.yml | 109 ++++++++++-------- .../baredsc/test-data/combined_1d_plot.png | Bin .../test-data/nih3t3_generated_2d_2.txt | 0 7 files changed, 64 insertions(+), 51 deletions(-) rename workflows/{transcriptomics => scRNAseq}/baredsc/.dockstore.yml (100%) rename workflows/{transcriptomics => scRNAseq}/baredsc/CHANGELOG.md (100%) rename workflows/{transcriptomics => scRNAseq}/baredsc/README.md (100%) rename workflows/{transcriptomics => scRNAseq}/baredsc/baredSC_1d_logNorm.ga (99%) rename workflows/{transcriptomics => scRNAseq}/baredsc/baredSC_1d_logNorm_tests.yml (61%) rename workflows/{transcriptomics => scRNAseq}/baredsc/test-data/combined_1d_plot.png (100%) rename workflows/{transcriptomics => scRNAseq}/baredsc/test-data/nih3t3_generated_2d_2.txt (100%) diff --git a/workflows/transcriptomics/baredsc/.dockstore.yml b/workflows/scRNAseq/baredsc/.dockstore.yml similarity index 100% rename from workflows/transcriptomics/baredsc/.dockstore.yml rename to workflows/scRNAseq/baredsc/.dockstore.yml diff --git a/workflows/transcriptomics/baredsc/CHANGELOG.md b/workflows/scRNAseq/baredsc/CHANGELOG.md similarity index 100% rename from workflows/transcriptomics/baredsc/CHANGELOG.md rename to workflows/scRNAseq/baredsc/CHANGELOG.md diff --git a/workflows/transcriptomics/baredsc/README.md b/workflows/scRNAseq/baredsc/README.md similarity index 100% rename from workflows/transcriptomics/baredsc/README.md rename to workflows/scRNAseq/baredsc/README.md diff --git a/workflows/transcriptomics/baredsc/baredSC_1d_logNorm.ga b/workflows/scRNAseq/baredsc/baredSC_1d_logNorm.ga similarity index 99% rename from workflows/transcriptomics/baredsc/baredSC_1d_logNorm.ga rename to workflows/scRNAseq/baredsc/baredSC_1d_logNorm.ga index 915dcf17d..ad47b90b9 100644 --- a/workflows/transcriptomics/baredsc/baredSC_1d_logNorm.ga +++ b/workflows/scRNAseq/baredsc/baredSC_1d_logNorm.ga @@ -74,11 +74,11 @@ "input_connections": {}, "inputs": [ { - "description": "Maximum value to explore in logNorm", - "name": "Maximum value in logNorm (usually between 2 and 4)" + "description": "Maximum value to explore in logNorm (usually between 2 and 4)", + "name": "Maximum value in logNorm" } ], - "label": "Maximum value in logNorm (usually between 2 and 4)", + "label": "Maximum value in logNorm", "name": "Input parameter", "outputs": [], "position": { diff --git a/workflows/transcriptomics/baredsc/baredSC_1d_logNorm_tests.yml b/workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml similarity index 61% rename from workflows/transcriptomics/baredsc/baredSC_1d_logNorm_tests.yml rename to workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml index 9894e4440..ab96f30da 100644 --- a/workflows/transcriptomics/baredsc/baredSC_1d_logNorm_tests.yml +++ b/workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml @@ -12,79 +12,94 @@ element_tests: split_file_000000.tabular: asserts: - size: 1257919 - delta_frac: 0.1 + has_size: + value: 1257919 + delta: 100000 split_file_000001.tabular: asserts: - size: 1601519 - delta_frac: 0.1 + has_size: + value: 1601519 + delta: 100000 split_file_000002.tabular: asserts: - size: 2180423 - delta_frac: 0.1 + has_size: + value: 2180423 + delta: 200000 split_file_000003.tabular: asserts: - size: 28234812 - delta_frac: 0.1 - split_file_000003.tabular: + has_size: + value: 28234812 + delta: 2000000 baredsc_qc_plots: element_tests: split_file_000000.tabular: element_tests: convergence: asserts: - size: 20304 - delta_frac: 0.1 + has_size: + value: 20304 + delta: 1000 corner: asserts: - size: 63603 - delta_frac: 0.1 + has_size: + value: 63603 + delta: 6000 p: asserts: - size: 45302 - delta_frac: 0.1 + has_size: + value: 45302 + delta: 4000 split_file_000001.tabular: element_tests: convergence: asserts: - size: 27704 - delta_frac: 0.1 + has_size: + value: 27704 + delta: 2000 corner: asserts: - size: 387365 - delta_frac: 0.1 + has_size: + value: 387365 + delta: 30000 p: asserts: - size: 97623 - delta_frac: 0.1 + has_size: + value: 97623 + delta: 90000 split_file_000002.tabular: element_tests: convergence: asserts: - size: 32463 - delta_frac: 0.1 + has_size: + value: 32463 + delta: 3000 corner: asserts: - size: 1103840 - delta_frac: 0.1 + has_size: + value: 1103840 + delta: 100000 p: asserts: - size: 159797 - delta_frac: 0.1 + has_size: + value: 159797 + delta: 10000 split_file_000003.tabular: element_tests: convergence: asserts: - size: 31343 - delta_frac: 0.1 + has_size: + value: 31343 + delta: 3000 corner: asserts: - size: 2139411 - delta_frac: 0.1 + has_size: + value: 2139411 + delta: 200000 p: asserts: - size: 175774 - delta_frac: 0.1 + has_size: + value: 175774 + delta: 10000 baredsc_neff: element_tests: split_file_000000.tabular: @@ -116,8 +131,8 @@ individuals: asserts: has_size: - size: 108407 - delta_frac: 0.1 + value: 108407 + delta: 10000 means: asserts: has_n_lines: @@ -127,34 +142,32 @@ posterior_andco: asserts: has_size: - size: 197980 - delta_frac: 0.1 + value: 197980 + delta: 10000 posterior_individuals: asserts: has_size: - size: 105262 - delta_frac: 0.1 + value: 105262 + delta: 10000 posterior_per_cell: asserts: has_n_lines: n: 2632 has_line_matching: - exoression: "mu\tsd" + expression: "mu\tsd" with_posterior: asserts: has_size: - size: 234303 - delta_frac: 0.1 + value: 234303 + delta: 20000 combined_pdf: asserts: - that: - - has_line: - line: "x\tlow\tmean\thigh\tmedian" - - has_text: - text: "0.0125\t" + has_line: + line: "x\tlow\tmean\thigh\tmedian" + has_text: + text: "0.0125\t" combined_plot: path: test-data/combined_1d_plot.png compare: sim_size delta_frac: 0.1 - \ No newline at end of file diff --git a/workflows/transcriptomics/baredsc/test-data/combined_1d_plot.png b/workflows/scRNAseq/baredsc/test-data/combined_1d_plot.png similarity index 100% rename from workflows/transcriptomics/baredsc/test-data/combined_1d_plot.png rename to workflows/scRNAseq/baredsc/test-data/combined_1d_plot.png diff --git a/workflows/transcriptomics/baredsc/test-data/nih3t3_generated_2d_2.txt b/workflows/scRNAseq/baredsc/test-data/nih3t3_generated_2d_2.txt similarity index 100% rename from workflows/transcriptomics/baredsc/test-data/nih3t3_generated_2d_2.txt rename to workflows/scRNAseq/baredsc/test-data/nih3t3_generated_2d_2.txt From 5b2266b53a46e794e3b8df8fc191f1cfa633dca7 Mon Sep 17 00:00:00 2001 From: Lucille Delisle Date: Tue, 3 Oct 2023 16:18:43 +0200 Subject: [PATCH 04/12] fix tests results --- workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml b/workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml index ab96f30da..acb23b291 100644 --- a/workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml +++ b/workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml @@ -125,7 +125,7 @@ has_n_lines: n: 1 has_line_matching: - expression: "78[0-9].[0-9]*" + expression: "[7-9][0-9][0-9].[0-9]*" combined_other_outputs: element_tests: individuals: @@ -152,7 +152,7 @@ posterior_per_cell: asserts: has_n_lines: - n: 2632 + n: 2362 has_line_matching: expression: "mu\tsd" with_posterior: From effe72e12273026fb7a429329aad5dcaf2153e56 Mon Sep 17 00:00:00 2001 From: Lucille Delisle Date: Thu, 12 Oct 2023 00:01:49 +0200 Subject: [PATCH 05/12] more lenient on test --- workflows/scRNAseq/baredsc/baredSC_1d_logNorm.ga | 2 +- workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml | 3 ++- 2 files changed, 3 insertions(+), 2 deletions(-) diff --git a/workflows/scRNAseq/baredsc/baredSC_1d_logNorm.ga b/workflows/scRNAseq/baredsc/baredSC_1d_logNorm.ga index ad47b90b9..3c70c46c0 100644 --- a/workflows/scRNAseq/baredsc/baredSC_1d_logNorm.ga +++ b/workflows/scRNAseq/baredsc/baredSC_1d_logNorm.ga @@ -1,6 +1,6 @@ { "a_galaxy_workflow": "true", - "annotation": "Run baredSC in logNorm for 1 to N gaussians and combine models.", + "annotation": "Run baredSC in 1 dimension in logNorm for 1 to N gaussians and combine models.", "creator": [ { "class": "Person", diff --git a/workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml b/workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml index acb23b291..4e1a1210a 100644 --- a/workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml +++ b/workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml @@ -119,7 +119,7 @@ has_n_lines: n: 1 has_line_matching: - expression: "29[0-9].[0-9]*" + expression: "2[0-9][0-9].[0-9]*" split_file_000003.tabular: asserts: has_n_lines: @@ -137,6 +137,7 @@ asserts: has_n_lines: n: 99998 + delta: 4000 has_line_matching: expression: "6.[0-9]*e-01" posterior_andco: From 8c45d6a864e25a2324a1c74bbfbe5c0081b5ae83 Mon Sep 17 00:00:00 2001 From: Lucille Delisle Date: Thu, 12 Oct 2023 00:02:22 +0200 Subject: [PATCH 06/12] prepare README for 2d --- workflows/scRNAseq/baredsc/README.md | 7 ++++++- 1 file changed, 6 insertions(+), 1 deletion(-) diff --git a/workflows/scRNAseq/baredsc/README.md b/workflows/scRNAseq/baredsc/README.md index 2e98be41a..1ca2498a4 100644 --- a/workflows/scRNAseq/baredsc/README.md +++ b/workflows/scRNAseq/baredsc/README.md @@ -4,14 +4,19 @@ These workflows allow to run a baredSC analysis in a single click. It uses model ## Inputs dataset -- The workflow needs a tabular dataset where each row is a cell. The tabular needs to have a header line with column names. There must be at least two columns: 'nCount_RNA' and another one with the counts for the gene of interest. +- Both workflows need a tabular dataset where each row is a cell. The tabular needs to have a header line with column names. There must be at least two columns: 'nCount_RNA' and another one with the counts for the gene(s) of interest. ## Inputs values +For the 1D: + - Gene name: The name of the column with the counts of your gene of interest. - Maximum value in logNorm: The maximum value to explore in PDF. This value should be large enough so the PDF is at 0 at this value. - Maximum number of Gaussians to study: All models between models with 1 Gaussians to models with this number of Gaussians. +For the 2D: + + ## Processing - The workflow will generate paramater values from 1 to the maximum number of Gaussians to study. From fef52dacde9fc24498e2f05b9c034718502996ba Mon Sep 17 00:00:00 2001 From: Lucille Delisle Date: Wed, 18 Oct 2023 07:39:28 +0200 Subject: [PATCH 07/12] add 2D --- workflows/scRNAseq/baredsc/README.md | 9 +- ...tests.yml => baredSC-1d-logNorm-tests.yml} | 0 ...SC_1d_logNorm.ga => baredSC-1d-logNorm.ga} | 1 + .../baredsc/baredSC-2d-logNorm-tests.yml | 170 +++++ .../scRNAseq/baredsc/baredSC-2d-logNorm.ga | 691 ++++++++++++++++++ .../baredsc/test-data/combined_2d_plot.png | Bin 0 -> 38074 bytes 6 files changed, 869 insertions(+), 2 deletions(-) rename workflows/scRNAseq/baredsc/{baredSC_1d_logNorm_tests.yml => baredSC-1d-logNorm-tests.yml} (100%) rename workflows/scRNAseq/baredsc/{baredSC_1d_logNorm.ga => baredSC-1d-logNorm.ga} (99%) create mode 100644 workflows/scRNAseq/baredsc/baredSC-2d-logNorm-tests.yml create mode 100644 workflows/scRNAseq/baredsc/baredSC-2d-logNorm.ga create mode 100644 workflows/scRNAseq/baredsc/test-data/combined_2d_plot.png diff --git a/workflows/scRNAseq/baredsc/README.md b/workflows/scRNAseq/baredsc/README.md index 1ca2498a4..e467dcbd3 100644 --- a/workflows/scRNAseq/baredsc/README.md +++ b/workflows/scRNAseq/baredsc/README.md @@ -1,6 +1,6 @@ # BaredSC Workflows -These workflows allow to run a baredSC analysis in a single click. It uses models from 1 to N Gaussians and combine them. +These workflows allow to run a baredSC analysis in a single click. It uses models from 1 to N Gaussians and combine them. It uses the logNorm scale, 100 bins for 1 dimension and 25 bins on each axis in 2 dimensions. ## Inputs dataset @@ -12,10 +12,15 @@ For the 1D: - Gene name: The name of the column with the counts of your gene of interest. - Maximum value in logNorm: The maximum value to explore in PDF. This value should be large enough so the PDF is at 0 at this value. -- Maximum number of Gaussians to study: All models between models with 1 Gaussians to models with this number of Gaussians. +- Maximum number of Gaussians to study: All models between models with 1 Gaussians to models with this number of Gaussians will be combined. For the 2D: +- Gene name for x axis: The name of the column with the counts of your gene in x axis. +- Gene name for y axis: The name of the column with the counts of your gene in y axis. +- maximum value in logNorm for x-axis: The maximum value to explore in PDF in the x axis. This value should be large enough so the PDF is at 0 at this value. +- maximum value in logNorm for y-axis: The maximum value to explore in PDF in the y axis. This value should be large enough so the PDF is at 0 at this value. +- Maximum number of Gaussians to study: All models between models with 1 2D-Gaussians to models with this number of 2D-Gaussians will be combined. ## Processing diff --git a/workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml b/workflows/scRNAseq/baredsc/baredSC-1d-logNorm-tests.yml similarity index 100% rename from workflows/scRNAseq/baredsc/baredSC_1d_logNorm_tests.yml rename to workflows/scRNAseq/baredsc/baredSC-1d-logNorm-tests.yml diff --git a/workflows/scRNAseq/baredsc/baredSC_1d_logNorm.ga b/workflows/scRNAseq/baredsc/baredSC-1d-logNorm.ga similarity index 99% rename from workflows/scRNAseq/baredsc/baredSC_1d_logNorm.ga rename to workflows/scRNAseq/baredsc/baredSC-1d-logNorm.ga index 3c70c46c0..d20e501a1 100644 --- a/workflows/scRNAseq/baredsc/baredSC_1d_logNorm.ga +++ b/workflows/scRNAseq/baredsc/baredSC-1d-logNorm.ga @@ -10,6 +10,7 @@ ], "format-version": "0.1", "license": "MIT", + "release": "0.1", "name": "baredSC_1d_logNorm", "steps": { "0": { diff --git a/workflows/scRNAseq/baredsc/baredSC-2d-logNorm-tests.yml b/workflows/scRNAseq/baredsc/baredSC-2d-logNorm-tests.yml new file mode 100644 index 000000000..a93829562 --- /dev/null +++ b/workflows/scRNAseq/baredsc/baredSC-2d-logNorm-tests.yml @@ -0,0 +1,170 @@ +- doc: Test outline for baredSC_2d + job: + Tabular with raw expression values: + class: File + path: test-data/nih3t3_generated_2d_2.txt + filetype: tabular + Gene name for x axis: 0.5_0_0_0.5_x + Gene name for y axis: 0.5_0_0_0.5_y + maximum value in logNorm for x-axis: '2.5' + maximum value in logNorm for y-axis: '2.5' + Maximum number of Gaussians to study: '4' + outputs: + baredsc_numpy: + element_tests: + split_file_000000.tabular: + asserts: + has_size: + value: 1654994 + delta: 100000 + split_file_000001.tabular: + asserts: + has_size: + value: 2650966 + delta: 200000 + split_file_000002.tabular: + asserts: + has_size: + value: 39060170 + delta: 3000000 + split_file_000003.tabular: + asserts: + has_size: + value: 49913268 + delta: 5000000 + baredsc_qc_plots: + element_tests: + split_file_000000.tabular: + element_tests: + convergence: + asserts: + has_size: + value: 24300 + delta: 2000 + corner: + asserts: + has_size: + value: 300500 + delta: 30000 + p: + asserts: + has_size: + value: 98200 + delta: 10000 + split_file_000001.tabular: + element_tests: + convergence: + convergence: + asserts: + has_size: + value: 28600 + delta: 2000 + corner: + asserts: + has_size: + value: 2000000 + delta: 200000 + p: + asserts: + has_size: + value: 203100 + delta: 20000 + split_file_000002.tabular: + element_tests: + convergence: + asserts: + has_size: + value: 30200 + delta: 3000 + corner: + asserts: + has_size: + value: 5100000 + delta: 500000 + p: + asserts: + has_size: + value: 269500 + delta: 20000 + split_file_000003.tabular: + element_tests: + convergence: + asserts: + has_size: + value: 31500 + delta: 3000 + corner: + asserts: + has_size: + value: 9400000 + delta: 900000 + p: + asserts: + has_size: + value: 373300 + delta: 30000 + baredsc_neff: + element_tests: + split_file_000000.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "26[0-9][0-9].[0-9]*" + split_file_000001.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "8[0-9][0-9].[0-9]*" + split_file_000002.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "10[0-9][0-9].[0-9]*" + split_file_000003.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "5[0-9][0-9].[0-9]*" + combined_other_outputs: + element_tests: + corr: + asserts: + has_n_lines: + n: 2 + has_text_matching: + expression: "0.89[0-9]*" + has_text: + text: "mean\tmedian\tlow\thigh" + individuals: + asserts: + has_size: + value: 54400 + delta: 5000 + median: + asserts: + has_size: + value: 37100 + delta: 3000 + combined_pdf2d: + asserts: + has_n_lines: + n: 26 + has_text: + text: "xy\t0.05\t0.15" + combined_pdf2d_flat: + asserts: + has_n_lines: + n: 626 + has_line: + line: "x\ty\tlow\tmean\thigh\tmedian" + has_text: + text: "0.05\t0.05" + combined_plot: + path: test-data/combined_2d_plot.png + compare: sim_size + delta_frac: 0.1 + \ No newline at end of file diff --git a/workflows/scRNAseq/baredsc/baredSC-2d-logNorm.ga b/workflows/scRNAseq/baredsc/baredSC-2d-logNorm.ga new file mode 100644 index 000000000..f0dd568e2 --- /dev/null +++ b/workflows/scRNAseq/baredsc/baredSC-2d-logNorm.ga @@ -0,0 +1,691 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "Run baredSC in 2 dimensions in logNorm for 1 to N gaussians and combine models.", + "creator": [ + { + "class": "Person", + "identifier": "https://orcid.org/0000-0002-1964-4960", + "name": "Lucille Delisle" + } + ], + "format-version": "0.1", + "license": "MIT", + "name": "baredSC_2d_logNorm", + "steps": { + "0": { + "annotation": "The dataset must have a first row with row names. 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z{Kq^z!9zo8z+YMd(*P+MnVsO1o5(G0O3;t}Iv{$zRYOhL=fFbfU4xwK3y}&EJ3G5Z zzc&sxwj#g6>YLUVEilZoVWUXB@FJaUu;EDM4^Rj!DyP==oB?*>y7Y#zK?L#m3WDAHI%RgLcZ_U7w zZ&<0r&cWdiP@e3(JoE(C=KvcZfWE8*WmFdlIeZho%bg*k}EkXZ0J zZ4kmZLwdQn^{uH&tmB>ePQdH1b8!U%CoZeDmJA4bks@ARUdrQ_K)e@qh2|){L)d!I z8ug=&j+{+QO_c-0plLqw#Weg`?Hi`Ye^FV0S)w9; ziw;Xj#>-3idPd>S1Nn)P7xakZNPB_X7jbNHwBy_hO1t*<8T&cy*gQEsKE7sm;V60x z_(%Szr3~K(TNiNgt*Lb@UfqFu%#4T#56|k}qq|@ZdHgc+T)|*xzBvjm zLv<9!vQCB=C$M- Date: Wed, 18 Oct 2023 07:59:39 +0200 Subject: [PATCH 08/12] update dockstore --- workflows/scRNAseq/baredsc/.dockstore.yml | 15 +++++++++++++-- 1 file changed, 13 insertions(+), 2 deletions(-) diff --git a/workflows/scRNAseq/baredsc/.dockstore.yml b/workflows/scRNAseq/baredsc/.dockstore.yml index 54602e951..3073aa73e 100644 --- a/workflows/scRNAseq/baredsc/.dockstore.yml +++ b/workflows/scRNAseq/baredsc/.dockstore.yml @@ -1,9 +1,20 @@ version: 1.2 workflows: -- name: main +- name: baredSC-1d-logNorm subclass: Galaxy publish: true - primaryDescriptorPath: /baredSC_1d_logNorm.ga + primaryDescriptorPath: /baredSC-1d-logNorm.ga + testParameterFiles: + - /baredSC-1d-logNorm-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 +- name: baredSC-2d-logNorm + subclass: Galaxy + publish: true + primaryDescriptorPath: /baredSC-2d-logNorm.ga + testParameterFiles: + - /baredSC-2d-logNorm-tests.yml authors: - name: Lucille Delisle orcid: 0000-0002-1964-4960 From 231db9bf4348976c3d50b0ef958680072ffbf6d0 Mon Sep 17 00:00:00 2001 From: Lucille Delisle Date: Wed, 18 Oct 2023 08:00:57 +0200 Subject: [PATCH 09/12] update baredsc --- .../scRNAseq/baredsc/baredSC-1d-logNorm.ga | 22 +++++++++---------- .../scRNAseq/baredsc/baredSC-2d-logNorm.ga | 21 +++++++++--------- 2 files changed, 22 insertions(+), 21 deletions(-) diff --git a/workflows/scRNAseq/baredsc/baredSC-1d-logNorm.ga b/workflows/scRNAseq/baredsc/baredSC-1d-logNorm.ga index d20e501a1..cc461debc 100644 --- a/workflows/scRNAseq/baredsc/baredSC-1d-logNorm.ga +++ b/workflows/scRNAseq/baredsc/baredSC-1d-logNorm.ga @@ -75,7 +75,7 @@ "input_connections": {}, "inputs": [ { - "description": "Maximum value to explore in logNorm (usually between 2 and 4)", + "description": "Maximum value to explore in logNorm", "name": "Maximum value in logNorm" } ], @@ -226,7 +226,7 @@ }, "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_text_file_with_recurring_lines/1.1.0", "tool_shed_repository": { - "changeset_revision": "ddf54b12c295", + "changeset_revision": "d698c222f354", "name": "text_processing", "owner": "bgruening", "tool_shed": "toolshed.g2.bx.psu.edu" @@ -402,7 +402,7 @@ } }, "tags": "", - "uuid": "4cfee916-a09d-424c-811e-220116374bf9" + "uuid": "7b292353-e3cc-4fa9-8898-b4d3e3ea4901" }, "tool_id": null, "type": "subworkflow", @@ -412,7 +412,7 @@ }, "5": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_1d/baredsc_1d/1.1.1+galaxy0", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_1d/baredsc_1d/1.1.2+galaxy0", "errors": null, "id": 5, "input_connections": { @@ -492,15 +492,15 @@ "output_name": "plot" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_1d/baredsc_1d/1.1.1+galaxy0", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_1d/baredsc_1d/1.1.2+galaxy0", "tool_shed_repository": { - "changeset_revision": "02b1fe7aed76", + "changeset_revision": "4fff5a293013", "name": "baredsc_1d", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, "tool_state": "{\"MCMC\": {\"xmin\": \"0.0\", \"xmax\": {\"__class__\": \"ConnectedValue\"}, \"nx\": \"100\", \"minScalex\": \"0.1\", \"scale\": {\"type\": \"Seurat\", \"__current_case__\": 0, \"targetSum\": \"10000.0\"}, \"seed\": \"1\", \"nnorm\": {\"__class__\": \"ConnectedValue\"}, \"nsampMCMC\": \"100000\", \"automaticRestart\": {\"set_minNeff\": \"yes\", \"__current_case__\": 0, \"minNeff\": \"200.0\"}}, \"advanced\": {\"osampx\": \"10\", \"osampxpdf\": \"5\", \"coviscale\": \"1.0\", \"nis\": \"1000\", \"burn\": {\"custom\": \"no\", \"__current_case__\": 0}}, \"filter\": {\"nb\": \"0\", \"__current_case__\": 0}, \"geneColName\": {\"__class__\": \"ConnectedValue\"}, \"input_counts\": {\"filetype\": \"tabular\", \"__current_case__\": 0, \"input\": {\"__class__\": \"ConnectedValue\"}}, \"plots\": {\"image_file_format\": \"png\", \"title\": \"\", \"removeFirstSamples\": \"-1\", \"nsampInPlot\": \"100000\", \"prettyBins\": \"-1\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "1.1.1+galaxy0", + "tool_version": "1.1.2+galaxy0", "type": "tool", "uuid": "75379c38-7040-4651-b875-5ad7382de31d", "when": null, @@ -524,7 +524,7 @@ }, "6": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_combine_1d/baredsc_combine_1d/1.1.1+galaxy0", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_combine_1d/baredsc_combine_1d/1.1.2+galaxy0", "errors": null, "id": 6, "input_connections": { @@ -567,15 +567,15 @@ "top": 170.5 }, "post_job_actions": {}, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_combine_1d/baredsc_combine_1d/1.1.1+galaxy0", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_combine_1d/baredsc_combine_1d/1.1.2+galaxy0", "tool_shed_repository": { - "changeset_revision": "c32347702e12", + "changeset_revision": "050cf43887d3", "name": "baredsc_combine_1d", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, "tool_state": "{\"MCMC\": {\"outputs\": {\"__class__\": \"ConnectedValue\"}, \"xmin\": \"0.0\", \"xmax\": {\"__class__\": \"ConnectedValue\"}, \"nx\": \"100\", \"minScalex\": \"0.1\", \"scale\": {\"type\": \"Seurat\", \"__current_case__\": 0, \"targetSum\": \"10000.0\"}, \"seed\": \"1\"}, \"advanced\": {\"osampx\": \"10\", \"osampxpdf\": \"5\", \"coviscale\": \"1.0\", \"nis\": \"1000\"}, \"filter\": {\"nb\": \"0\", \"__current_case__\": 0}, \"geneColName\": {\"__class__\": \"ConnectedValue\"}, \"input_counts\": {\"filetype\": \"tabular\", \"__current_case__\": 0, \"input\": {\"__class__\": \"ConnectedValue\"}}, \"plots\": {\"image_file_format\": \"png\", \"title\": \"\", \"removeFirstSamples\": \"-1\", \"nsampInPlot\": \"100000\", \"prettyBins\": \"-1\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "1.1.1+galaxy0", + "tool_version": "1.1.2+galaxy0", "type": "tool", "uuid": "752ba293-93e9-4769-85d0-ebc9c2c100b6", "when": null, diff --git a/workflows/scRNAseq/baredsc/baredSC-2d-logNorm.ga b/workflows/scRNAseq/baredsc/baredSC-2d-logNorm.ga index f0dd568e2..1c562e790 100644 --- a/workflows/scRNAseq/baredsc/baredSC-2d-logNorm.ga +++ b/workflows/scRNAseq/baredsc/baredSC-2d-logNorm.ga @@ -10,6 +10,7 @@ ], "format-version": "0.1", "license": "MIT", + "release": "0.1", "name": "baredSC_2d_logNorm", "steps": { "0": { @@ -279,7 +280,7 @@ }, "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_text_file_with_recurring_lines/1.1.0", "tool_shed_repository": { - "changeset_revision": "ddf54b12c295", + "changeset_revision": "d698c222f354", "name": "text_processing", "owner": "bgruening", "tool_shed": "toolshed.g2.bx.psu.edu" @@ -455,7 +456,7 @@ } }, "tags": "", - "uuid": "4cfee916-a09d-424c-811e-220116374bf9" + "uuid": "63ecefb0-2276-454b-93d0-ee83f3662157" }, "tool_id": null, "type": "subworkflow", @@ -465,7 +466,7 @@ }, "7": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_2d/baredsc_2d/1.1.1+galaxy0", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_2d/baredsc_2d/1.1.2+galaxy0", "errors": null, "id": 7, "input_connections": { @@ -562,15 +563,15 @@ "output_name": "plot" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_2d/baredsc_2d/1.1.1+galaxy0", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_2d/baredsc_2d/1.1.2+galaxy0", "tool_shed_repository": { - "changeset_revision": "c29e7bb492bf", + "changeset_revision": "aa4a6f0916b4", "name": "baredsc_2d", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, "tool_state": "{\"MCMC\": {\"xmin\": \"0.0\", \"xmax\": {\"__class__\": \"ConnectedValue\"}, \"nx\": \"25\", \"minScalex\": \"0.1\", \"ymin\": \"0.0\", \"ymax\": {\"__class__\": \"ConnectedValue\"}, \"ny\": \"25\", \"minScaley\": \"0.1\", \"scale\": {\"type\": \"Seurat\", \"__current_case__\": 0, \"targetSum\": \"10000.0\"}, \"seed\": \"1\", \"nnorm\": {\"__class__\": \"ConnectedValue\"}, \"nsampMCMC\": \"100000\", \"automaticRestart\": {\"set_minNeff\": \"yes\", \"__current_case__\": 0, \"minNeff\": \"200.0\"}}, \"advanced\": {\"osampx\": \"10\", \"osampxpdf\": \"4\", \"osampy\": \"10\", \"osampypdf\": \"4\", \"coviscale\": \"1.0\", \"nis\": \"1000\", \"burn\": {\"custom\": \"no\", \"__current_case__\": 0}, \"scalePrior\": \"0.3\"}, \"filter\": {\"nb\": \"0\", \"__current_case__\": 0}, \"geneXColName\": {\"__class__\": \"ConnectedValue\"}, \"geneYColName\": {\"__class__\": \"ConnectedValue\"}, \"input_counts\": {\"filetype\": \"tabular\", \"__current_case__\": 0, \"input\": {\"__class__\": \"ConnectedValue\"}}, \"plots\": {\"image_file_format\": \"png\", \"title\": \"\", \"removeFirstSamples\": \"-1\", \"nsampInPlot\": \"100000\", \"prettyBinsx\": \"-1\", \"prettyBinsy\": \"-1\", \"log1pColorScale\": false, \"splity\": \"\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "1.1.1+galaxy0", + "tool_version": "1.1.2+galaxy0", "type": "tool", "uuid": "3fb4de69-9b40-4243-92fb-75553ff8ef09", "when": null, @@ -594,7 +595,7 @@ }, "8": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_combine_2d/baredsc_combine_2d/1.1.1+galaxy0", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_combine_2d/baredsc_combine_2d/1.1.2+galaxy0", "errors": null, "id": 8, "input_connections": { @@ -649,15 +650,15 @@ "top": 199.5 }, "post_job_actions": {}, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_combine_2d/baredsc_combine_2d/1.1.1+galaxy0", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/baredsc_combine_2d/baredsc_combine_2d/1.1.2+galaxy0", "tool_shed_repository": { - "changeset_revision": "239193b96d72", + "changeset_revision": "ae969fdb8442", "name": "baredsc_combine_2d", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, "tool_state": "{\"MCMC\": {\"outputs\": {\"__class__\": \"ConnectedValue\"}, \"xmin\": \"0.0\", \"xmax\": {\"__class__\": \"ConnectedValue\"}, \"nx\": \"25\", \"minScalex\": \"0.1\", \"ymin\": \"0.0\", \"ymax\": {\"__class__\": \"ConnectedValue\"}, \"ny\": \"25\", \"minScaley\": \"0.1\", \"scale\": {\"type\": \"Seurat\", \"__current_case__\": 0, \"targetSum\": \"10000.0\"}, \"seed\": \"1\"}, \"advanced\": {\"osampx\": \"10\", \"osampxpdf\": \"4\", \"osampy\": \"10\", \"osampypdf\": \"4\", \"coviscale\": \"1.0\", \"nis\": \"1000\", \"scalePrior\": \"0.3\"}, \"filter\": {\"nb\": \"0\", \"__current_case__\": 0}, \"geneXColName\": {\"__class__\": \"ConnectedValue\"}, \"geneYColName\": {\"__class__\": \"ConnectedValue\"}, \"input_counts\": {\"filetype\": \"tabular\", \"__current_case__\": 0, \"input\": {\"__class__\": \"ConnectedValue\"}}, \"plots\": {\"image_file_format\": \"png\", \"title\": \"\", \"removeFirstSamples\": \"-1\", \"nsampInPlot\": \"100000\", \"prettyBinsx\": \"-1\", \"prettyBinsy\": \"-1\", \"log1pColorScale\": false, \"splity\": \"\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "1.1.1+galaxy0", + "tool_version": "1.1.2+galaxy0", "type": "tool", "uuid": "e4eebae4-0952-4d21-8bb6-d16d1b5397b3", "when": null, From f8dacfb21817e1720329b03b6dd4d186f952e7cb Mon Sep 17 00:00:00 2001 From: Lucille Delisle Date: Wed, 18 Oct 2023 15:39:08 +0200 Subject: [PATCH 10/12] more lenient on neff --- workflows/scRNAseq/baredsc/baredSC-2d-logNorm-tests.yml | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/workflows/scRNAseq/baredsc/baredSC-2d-logNorm-tests.yml b/workflows/scRNAseq/baredsc/baredSC-2d-logNorm-tests.yml index a93829562..c6a8b61fb 100644 --- a/workflows/scRNAseq/baredsc/baredSC-2d-logNorm-tests.yml +++ b/workflows/scRNAseq/baredsc/baredSC-2d-logNorm-tests.yml @@ -110,25 +110,25 @@ has_n_lines: n: 1 has_line_matching: - expression: "26[0-9][0-9].[0-9]*" + expression: "[1-2]?[0-9][0-9][0-9].[0-9]*" split_file_000001.tabular: asserts: has_n_lines: n: 1 has_line_matching: - expression: "8[0-9][0-9].[0-9]*" + expression: "[1-2]?[0-9][0-9][0-9].[0-9]*" split_file_000002.tabular: asserts: has_n_lines: n: 1 has_line_matching: - expression: "10[0-9][0-9].[0-9]*" + expression: "[1-2]?[0-9][0-9][0-9].[0-9]*" split_file_000003.tabular: asserts: has_n_lines: n: 1 has_line_matching: - expression: "5[0-9][0-9].[0-9]*" + expression: "[1-2]?[0-9][0-9][0-9].[0-9]*" combined_other_outputs: element_tests: corr: From 224a0a3efd91e0c182ea925f0d0084bec1141c7b Mon Sep 17 00:00:00 2001 From: Lucille Delisle Date: Fri, 20 Oct 2023 09:52:37 +0200 Subject: [PATCH 11/12] add info how to generate input --- workflows/scRNAseq/baredsc/README.md | 12 ++++++++++-- 1 file changed, 10 insertions(+), 2 deletions(-) diff --git a/workflows/scRNAseq/baredsc/README.md b/workflows/scRNAseq/baredsc/README.md index e467dcbd3..b8f06955d 100644 --- a/workflows/scRNAseq/baredsc/README.md +++ b/workflows/scRNAseq/baredsc/README.md @@ -1,10 +1,18 @@ # BaredSC Workflows -These workflows allow to run a baredSC analysis in a single click. It uses models from 1 to N Gaussians and combine them. It uses the logNorm scale, 100 bins for 1 dimension and 25 bins on each axis in 2 dimensions. +These workflows allow to run a baredSC analysis from a table with counts in a single click. It uses models from 1 to N Gaussians and combine them. It uses the logNorm scale, 100 bins for 1 dimension and 25 bins on each axis in 2 dimensions. ## Inputs dataset -- Both workflows need a tabular dataset where each row is a cell. The tabular needs to have a header line with column names. There must be at least two columns: 'nCount_RNA' and another one with the counts for the gene(s) of interest. +- Both workflows need a tabular dataset where each row is a cell. The tabular needs to have a header line with column names. There must be at least two columns: 'nCount_RNA' and another one with the counts for the gene(s) of interest. A way to get such table in R from a Seurat object (`seurat.obj`) is: + +```r +my.genes <- c("Hoxa13", "Hoxd13") +df <- cbind(seurat.obj[[]], # This will give you all metadata including nCount_RNA + FetchData(seurat.obj, slot = "counts", vars = my.genes)) + +write.table(df, "input_for_baredSC.txt", quote = F, sep = "\t", row.names = F) +``` ## Inputs values From 51106ce6545bda16b7b2ead5a1e50f99f329781b Mon Sep 17 00:00:00 2001 From: Lucille Delisle Date: Fri, 20 Oct 2023 09:55:18 +0200 Subject: [PATCH 12/12] fix README [skip ci] --- workflows/scRNAseq/baredsc/README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/scRNAseq/baredsc/README.md b/workflows/scRNAseq/baredsc/README.md index b8f06955d..1331dfa85 100644 --- a/workflows/scRNAseq/baredsc/README.md +++ b/workflows/scRNAseq/baredsc/README.md @@ -33,5 +33,5 @@ For the 2D: ## Processing - The workflow will generate paramater values from 1 to the maximum number of Gaussians to study. -- baredSC_1d is run for each of these number of Gaussians +- baredSC_1d or baredSC_2d is run for each of these number of Gaussians - All models are combined into a single result.