Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

error during aligning #160

Open
safisher opened this issue Jan 4, 2013 · 0 comments
Open

error during aligning #160

safisher opened this issue Jan 4, 2013 · 0 comments

Comments

@safisher
Copy link

safisher commented Jan 4, 2013

I received the following error when aligning. I tried to rerun the RUM job and RUM tells me that my config file has the output director set to ".". However, the config file clearly has the output directory properly set. The config file is also included below.


Thu Jan 3 18:34:25 2013 59706 FATAL RUM::Death - Can't close(GLOB(0x491268b8)) filehandle: '' at /home/fisher/local/src/RUM-Pipeline-v2.0.3_04/bin/../lib/RUM/Script/SortRumByLocation.pm line 271
Thu Jan 3 18:34:32 2013 46723 FATAL RUM::Death -
Error running "perl /home/fisher/local/src/RUM-Pipeline-v2.0.3_04/bin/../bin/sort_RUM_by_location.pl --ram 6 /data12/fisher/Sample_H0D11/rum.trim/chunks/RUM_NU.16 -o /data12/fisher/Sample_H0D11/rum.trim/chunks/RUM_NU.sorted.16.tmp.Dm_CmqHW >> /data12/fisher/Sample_H0D11/rum.trim/chunks/chr_counts_nu.16.tmp._IJnR6PX"

The stderr from that command is

Can't close(GLOB(0x491268b8)) filehandle: '' at /home/fisher/local/src/RUM-Pipeline-v2.0.3_04/bin/../lib/RUM/Script/SortRumByLocation.pm line 271

The error log file Sample_H0D11/rum.trim/log/rum_errors_16.log may have more details.


$VAR1 = bless( {
'version' => 'v2.0.3_04',
'name' => 'Sample_H0D11',
'reverse_reads' => 'Sample_H0D11/trimAT/unaligned_2.fq',
'output_dir' => '/data12/fisher/Sample_H0D11/rum.trim',
'chunks' => '48',
'paired_end' => 1,
'variable_length_reads' => 1,
'forward_reads' => 'Sample_H0D11/trimAT/unaligned_1.fq',
'index_dir' => '/lab/repo/resources/rum2/drosophila',
'_default' => bless( {
'bowtie_nu_limit' => 100,
'blat_rep_match' => 256,
'blat_max_intron' => 500000,
'max_insertions' => 1,
'blat_tile_size' => 12,
'blat_min_identity' => 93,
'platform' => 'Local',
'blat_step_size' => 6
}, 'RUM::Config' ),
'ram_ok' => 1,
'read_length' => 'v',
'ram' => 6
}, 'RUM::Config' );

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant