From f5dc28acce57e588296506889870f815330a8525 Mon Sep 17 00:00:00 2001
From: Joyjit Daw <1127155+tijyojwad@users.noreply.github.com>
Date: Tue, 10 Oct 2023 13:19:16 +0100
Subject: [PATCH] [README] fix samtools split cmd

---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 791ad5017..788056a62 100644
--- a/README.md
+++ b/README.md
@@ -164,7 +164,7 @@ The default heuristic for double-ended barcodes is to look for them on either en
 The output from `dorado basecaller` can be demultiplexed into per-barcode BAMs using `samtools split`. e.g.
 
 ```
-$ samtools split -u <basecalled-bam> -f <output-dir>/<prefix>_%!.bam
+$ samtools split -u <output-dir>/unclassified.bam -f "<output-dir>/<prefix>_%!.bam" <input-bam>
 ```
 
 #### Classifying existing datasets