Ambient RNA removal and doublet detection for Smart-seq2 dataset

[ngvananh2508]

Question

I have a set of 4 Smart-seq2 sc-RNA seq datasets. Should I do the ambient RNA removal and doublet dectection steps? In the tutorial, two steps above are mentioned for droplet-based platform? And should I do the quality control, normalization, feature selection steps before conducting data integration. If doing these steps, should I do it separately for each dataset or combine them then do this?

Thank you very much for helping me.

[Rohit-Satyam]

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