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In the Single cell best practices DGE analysis, it is mentioned:
"Volcano plots are often used to visualize the results of statistical testing, and they show the change in expression on the x-axis (log-fold change) and statistical significance on the y-axis (FDR-corrected p-values)."
But in the volcano plot code, pvalue was used to plot on the y axis.
ramadatta
changed the title
Question about the tutorial
pvalues for volcano plots instead of FDR-corrected p-values
Aug 21, 2024
ramadatta
changed the title
pvalues for volcano plots instead of FDR-corrected p-values
Usage of pvals for volcano plots instead of FDR-corrected p-values
Aug 21, 2024
Question
In the Single cell best practices DGE analysis, it is mentioned:
But in the volcano plot code, pvalue was used to plot on the y axis.
result["-logQ"] = -np.log(result["pvals"].astype("float"))
It should instead be pvals_adj (since
sc_toolbox.tools.de_res_to_anndata
renames FDR to pvals_adj)I found transition from FDR -> pvals_adj -> pvals a bit confusing. Is there some specific reason, please let me know if I missing something.
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