The latest version of AutoPrimer3 fixes issues arising from UCSC's recent update to their genome metadata that stopped previous versions from working. It also gives the option to specify names for targets when designing to regions/coordinates.
AutoPrimer3 retrieves gene information, DNA sequences and SNP information from the UCSC genome browser and uses primer3 to automatically design primers to genes or genomic coordinate targets.
Primers may be designed using information from any of the UCSC hosted genomes and primers can be made to avoid overlapping SNPs for genomes where SNP databases are available.
An instructions PDF can be viewed by selecting the 'Help' menu item from the application or by visiting here.
Get the latest release here.
AutoPrimer3 was written by David A. Parry ([email protected])
If you use this program to generate primers used in a publication, please cite the URL 'https://github.com/david-a-parry/autoprimer3'.
More information about Primer3, the primer design tool employed by AutoPrimer3, is available at http://primer3.sourceforge.net.
AutoPrimer3 is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.
This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.
You should have received a copy of the GNU General Public License along with this program. If not, see http://www.gnu.org/licenses/.