Multiomics bioinformaticist · CITE-seq · ChIP-seq · bulk RNA-seq · genomics
10 years of wet-lab background → full-time bioinformatics since 2023
| Domain | Technologies |
|---|---|
| Single-cell CITE-seq | Seurat, Signac · GEX + ADT integration · cell type annotation |
| Single-cell atlases | Harmony · cross-dataset integration |
| ChIP-seq | DiffBind, ChIPseeker · sorted cell populations · histone profiling |
| Bulk RNA-seq | DESeq2, edgeR · differential expression · GSEA |
| Genomics | Neoantigen prediction · association studies · variant annotation |
R (primary): Seurat · Signac · DESeq2 · edgeR · ChIPseeker · DiffBind · ggplot2 · Bioconductor
Python: Scanpy · AnnData · pandas
Workflow & repro: bash · SLURM · renv · Quarto
My laboratory experience spans cell biology, immunology, molecular biology, microbiology, parasitology, and preclinical research, including:
- Cellular assays: In vitro cell culture, viability, cytotoxicity, apoptosis (Annexin V/PI), MTT, and Giemsa staining.
- Immunological techniques: Flow cytometry, ELISA, and immune response assessment.
- Molecular biology: Western blotting, RT-qPCR, and digital PCR.
- Microbiology & parasitology: Bacterial and fungal cultures, Staphylococcus aureus, nasal vaccine research, and Leishmania infantum studies.
- In vivo research: Animal models, toxicity assessment, and preclinical experimental studies.
This experimental background drives my approach to bioinformatics, with a strong focus on biological context, experimental understanding, and meaningful interpretation — not just statistical outputs.
📧 estevaocarlos.silvabarcelos@collaboratori.unipg.it
🔗 ORCID 0000-0001-7076-6165
🏛️ Università degli Studi di Perugia, Italy