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Pycytominer training - #7060

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beatrizserrano merged 27 commits into
galaxyproject:mainfrom
rmassei:pycitominer-training
Oct 8, 2026
Merged

beatrizserrano merged 27 commits into
galaxyproject:mainfrom
rmassei:pycitominer-training

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@rmassei

@rmassei rmassei commented Sep 2, 2026 •

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This PR adds a new hands-on tutorial to show how to use Pycytominer tool suite in Galaxy

File location: topics/imaging/tutorials/pycitominer/workflows/test-data
What this tutorial covers

  • run pycytominer in Galaxy
  • include all the main 5 steps of pycytominer

closes FAIR-imaging/galaxy-image-community#76

@d33bs d33bs left a comment

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Exciting, thanks for creating this @rmassei !

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Comment thread topics/imaging/tutorials/pycytominer/tutorial.bib
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@axiomcura axiomcura left a comment

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Really cool, thanks for developing this! I left some comments. Please feel free to ping me if you have any questions c:

Awesome work, kudos!

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@rmassei rmassei changed the title Pycitominer training Pycytominer training Sep 29, 2026
@rmassei

rmassei commented Sep 29, 2026 •

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Thanks a lot @d33bs and @axiomcura for your comments are reviews!
I addressed them in the new commits, especially the Pycytominer spelling issues and your other comments :)

@dianichj dianichj left a comment

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Awesome tutorial and great work! Very easy to follow. Here is my round of comments and suggestions.

Comment thread topics/imaging/tutorials/pycitominer/tutorial.md Outdated
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Comment thread topics/imaging/tutorials/pycytominer/tutorial.md
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We have now a table with just 4 lines, collapsed in the selected unique conditions!

![05-consensus.png](../../images/pycitominer/05-consensus.png)

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same suggestions as above

Comment thread topics/imaging/tutorials/pycitominer/tutorial.md Outdated
Comment thread topics/imaging/tutorials/pycitominer/tutorial.md Outdated

> <hands-on-title> Extract Pycytominer workflow from history </hands-on-title>
> 1. Now we can extract the workflow for batch processing:
> - Name it "pycytominer-full-steps".

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{% snippet faqs/galaxy/workflows_extract_from_history.md %}

I would suggest to add the snippet here, right below item 1, and then the naming part.

> <hands-on-title> Extract Pycytominer workflow from history </hands-on-title>
> 1. Now we can extract the workflow for batch processing:
> - Name it "pycytominer-full-steps".
> - Don't treat `01_platemap.tsv` and `01_single_cells.tsv` as inputs (the workflow is supposed to be applied to the image-based profiles directly).

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This step has been confusing in other tutorials. So based on the interface we can say "uncheck."

@dianichj dianichj left a comment

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Awesome tutorial and great work! Very easy to follow. Here is my round of comments and suggestions.

@beatrizserrano
beatrizserrano merged commit 917e68d into galaxyproject:main Oct 8, 2026
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[training]: finalize the pycytominer training

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