Automated Bash pipeline for fetching NCBI FASTA gene sequences, calculating sequence statistics, and generating reverse complement sequences for multiple gene accession IDs.
- Automatically downloads FASTA sequences from NCBI using Accession IDs.
- Calculates GC/AT percentage, N50 value, and sequence length.
- Computes window-based GC content statistics.
- Generates reverse complement sequences.
Run the script with any NCBI gene accession ID (e.g.,'NM_000059', NM_0000545, NM_000786, NM_000799):
cat > README.md << 'EOF'
Automated Bash pipeline for fetching NCBI FASTA gene sequences, calculating sequence statistics, and generating reverse complement sequences for multiple gene accession IDs.
- Automatically downloads FASTA sequences from NCBI using Accession IDs.
- Calculates GC/AT percentage, N50 value, and sequence length.
- Computes window-based GC content statistics.
- Generates reverse complement sequences.
Run the script with any NCBI gene accession ID (e.g.,'NM_000059', NM_0000545, NM_000786, NM_000799):